This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Joshua Sakon, the director of the X-ray Core facility, has established a very comprehensive program in structural biology in the COBRE Center. He solved the X-ray crystal structure of the collagen binding domain which served as the basis for development of the parathyroid hormone-collagen binding domain (PTH-CBD) fusion protein for treatment of osteoporosis. The X-ray core facility includes a Rigagku Cu anode generator with a Saturn 99 CCD detector, a Rigaku Cr anode generator with R-AXIS IV detector, a Robotic Protein crystallization instrument, and computer cluster graphics systems. Of the total cost of $1,298,000, COBRE provided $390,000 while the remainder was funded with grants from DOD-Army, DOE, NREL, NIH-BRIN, and ABI. The University has committed institutional funds for permanent staff electronics specialists Jerry Homesley and K.Z. Shein to maintain these instruments. While many of the protein crystal structures will be completely solved in house using the new X-ray diffraction instrumentation, the larger, more difficult protein structures will require collection of diffraction data at national synchrotron facilities. Joshua Sakon is making extensive use of these national synchrotron beam lines, and is a registered user of the Advanced Photon Source at the Argonne National Laboratory.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Center Core Grants (P30)
Project #
5P30RR031154-02
Application #
8364930
Study Section
Special Emphasis Panel (ZRR1-RI-2 (01))
Project Start
2011-06-01
Project End
2012-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
2
Fiscal Year
2011
Total Cost
$107,378
Indirect Cost
Name
University of Arkansas at Fayetteville
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
191429745
City
Fayetteville
State
AR
Country
United States
Zip Code
72701
Paracha, Sadia; Hestekin, Christa (2016) Field amplified sample stacking of amyloid beta (1-42) oligomers using capillary electrophoresis. Biomicrofluidics 10:033105
Gao, Feng; Kight, Alicia D; Henderson, Rory et al. (2015) Regulation of Structural Dynamics within a Signal Recognition Particle Promotes Binding of Protein Targeting Substrates. J Biol Chem 290:15462-74
Katikaneni, Ranjitha; Ponnapakkam, Tulasi; Matsushita, Osamu et al. (2014) Treatment and prevention of chemotherapy-induced alopecia with PTH-CBD, a collagen-targeted parathyroid hormone analog, in a non-depilated mouse model. Anticancer Drugs 25:30-8
Bajpai, Geetika; Simmen, Rosalia C M; Stenken, Julie A (2014) In vivo microdialysis sampling of adipokines CCL2, IL-6, and leptin in the mammary fat pad of adult female rats. Mol Biosyst 10:806-12
Stratford Jr, Robert; Vu, Christopher; Sakon, Joshua et al. (2014) Pharmacokinetics in rats of a long-acting human parathyroid hormone-collagen binding domain peptide construct. J Pharm Sci 103:768-75
Pryor, N Elizabeth; Moss, Melissa A; Hestekin, Christa N (2014) Capillary electrophoresis for the analysis of the effect of sample preparation on early stages of Aβ1-40 aggregation. Electrophoresis 35:1814-20
Ponnapakkam, T; Katikaneni, R; Sakon, J et al. (2014) Treating osteoporosis by targeting parathyroid hormone to bone. Drug Discov Today 19:204-8
Alwarsh, Sefat; Ayinuola, Kolawole; Dormi, Silvana S et al. (2013) Intercepting the Breslow intermediate via Claisen rearrangement: synthesis of complex tertiary alcohols without organometallic reagents. Org Lett 15:3-5
Horne, Alexandra J; Lessner, Daniel J (2013) Assessment of the oxidant tolerance of Methanosarcina acetivorans. FEMS Microbiol Lett 343:13-9
Krothapalli, Sowmya; May, Michael K; Hestekin, Christa N (2012) Capillary electrophoresis-single strand conformation polymorphism for the detection of multiple mutations leading to tuberculosis drug resistance. J Microbiol Methods 91:147-54

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