This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Tissue Processing and Analysis Core (TPAC) facility provides the WVU research community with technical services and material support for light and electron microscopic studies. The Core contains designated areas for tissue fixation, including animal perfusion fixation, as well as surgical microscopes for tissue dissection and a variety of microtomes and cryotomes for sectioning. Users receive services regarding all aspects of study design and tissue processing, as well as troubleshooting procedures and visualization techniques. Specific services offered range from routine histology services (frozen, plastic or paraffin sectioning), H&E staining, etc., and extend to immunocytochemistry, visualization of neuronal tract tracers, in situ hybridization, tissue microarray and TUNEL assays. Preparation of tissues for electron microscopy and ultra-thin sectioning services is also offered to facility users. Human brain tissues, obtained either in the course of surgical resections or from fresh cadaveric material is also available for researchers. A unique resource within the State of West Virginia, the TPAC offers multiple options for investigators to generate 3-D visualizations of their processed material. The TPAC is Co-Directed by two faculty members with substantial experience in light and electron microscopic histological techniques, and staffed by experienced and certified histotechnologists. Part of the TPAC is housed in newly constructed laboratory space in the Erma Byrd Biomedical Sciences Research Center.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Center Core Grants (P30)
Project #
5P30RR031155-02
Application #
8364960
Study Section
Special Emphasis Panel (ZRR1-RI-2 (01))
Project Start
2011-07-01
Project End
2012-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
2
Fiscal Year
2011
Total Cost
$97,899
Indirect Cost
Name
West Virginia University
Department
Otolaryngology
Type
Schools of Medicine
DUNS #
191510239
City
Morgantown
State
WV
Country
United States
Zip Code
26506
Nichols, Cody E; Shepherd, Danielle L; Knuckles, Travis L et al. (2015) Cardiac and mitochondrial dysfunction following acute pulmonary exposure to mountaintop removal mining particulate matter. Am J Physiol Heart Circ Physiol 309:H2017-30
Thapa, Dharendra; Nichols, Cody E; Lewis, Sara E et al. (2015) Transgenic overexpression of mitofilin attenuates diabetes mellitus-associated cardiac and mitochondria dysfunction. J Mol Cell Cardiol 79:212-23
Hindman, Bridget; Goeckeler, Zoe; Sierros, Kostas et al. (2015) Non-Muscle Myosin II Isoforms Have Different Functions in Matrix Rearrangement by MDA-MB-231 Cells. PLoS One 10:e0131920
Armstead, Andrea L; Arena, Christopher B; Li, Bingyun (2014) Exploring the potential role of tungsten carbide cobalt (WC-Co) nanoparticle internalization in observed toxicity toward lung epithelial cells in vitro. Toxicol Appl Pharmacol 278:1-8
Sinha, Satyabrata; Belcastro, Marycharmain; Datta, Poppy et al. (2014) Essential role of the chaperonin CCT in rod outer segment biogenesis. Invest Ophthalmol Vis Sci 55:3775-85
Gao, Xueli; Sinha, Satyabrata; Belcastro, Marycharmain et al. (2013) Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins. J Biol Chem 288:25760-8
Pistilli, Emidio E; Guo, Ge; Stauber, William T (2013) IL-15R? deficiency leads to mitochondrial and myofiber differences in fast mouse muscles. Cytokine 61:41-5
Zhang, Na; Zhang, Lijuan; Qiu, Bensheng et al. (2012) Correlation of volume transfer coefficient Ktrans with histopathologic grades of gliomas. J Magn Reson Imaging 36:355-63
Marrs, Glen S; Spirou, George A (2012) Embryonic assembly of auditory circuits: spiral ganglion and brainstem. J Physiol 590:2391-408
Belcastro, Marycharmain; Song, Hongman; Sinha, Satyabrata et al. (2012) Phosphorylation of phosducin accelerates rod recovery from transducin translocation. Invest Ophthalmol Vis Sci 53:3084-91

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