This proposal describes the continuation of the National Resource for Aplysia, the overall goal of which to provide consistently high-quality cultured sea hares, A. californica to researchers. A. californica is an important invertebrate (Opisthobranch mollusk) model system for health-related research, primarily in the neurophysiology and molecular biology of behavior and learning. We will continue to produce animals for research and to conduct basic research aimed at exploring new model uses and at improving the resource. The two aims of this project are: 1. Resource Production - Sales of animals to researchers in the US and internationally are projected to continue at present levels of 15,000 to 20,000 animals per year. This resource makes available A. californica at all life stages from eggs through mature adults by rearing animals in carefully controlled laboratory conditions. All life-stages are available in sufficient quantities to meet user demand throughout the year, eliminating reliance on wild-collected animals that have limited seasonal availability and are of unknown age and health status. The Resource also offers customized services for raising same-aged cohorts, using specific diets and harvesting tissues. New products to be offered in the coming grant period will include behaviorally validated mature and senescent animals. 2. Research Programs to support the Resource - We will extend two research projects initiated during the current grant period to create new Aplysia resources that we believe will expand the usefulness of this model system to researchers (1) Refinement of stages of aging to expand our understanding of stages of aging in the Aplysia model system and allow us to offer a more complete series of age-staged animals to our users wishing to study age-related phenomena and (2) Assessment of environmental effects on phenotype to investigate several approaches for culturing animals that somewhat mimic conditions in the wild.
The California sea hare, A. californica, is a large marine snail that has proven to be an outstanding model for studies of how the nervous system functions, particularly in memory and learning as well as in understanding human diseases that affect these functions. The National Resource for Aplysia raises and ships 15,000 to 20,000 of these animals per year to research laboratories. This proposal would continue the production of these animals and expand their usefulness for studies of aging.
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|Kempsell, Andrew T; Fieber, Lynne A (2015) Age-related deficits in synaptic plasticity rescued by activating PKA or PKC in sensory neurons of Aplysia californica. Front Aging Neurosci 7:173|
|Kempsell, Andrew T; Fieber, Lynne A (2015) Aging in Sensory and Motor Neurons Results in Learning Failure in Aplysia californica. PLoS One 10:e0127056|
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|Kempsell, Andrew T; Fieber, Lynne A (2014) Behavioral aging is associated with reduced sensory neuron excitability in Aplysia californica. Front Aging Neurosci 6:84|
|Lanni, Eric J; Dunham, Sage J B; Nemes, Peter et al. (2014) Biomolecular imaging with a C60-SIMS/MALDI dual ion source hybrid mass spectrometer: instrumentation, matrix enhancement, and single cell analysis. J Am Soc Mass Spectrom 25:1897-907|
|Wang, Wentian; Simovic, Dragan D; Di, Mingping et al. (2013) Synthesis, receptor binding and activity of iso and azakainoids. Bioorg Med Chem Lett 23:1949-52|
|Kadakkuzha, Beena M; Akhmedov, Komolitdin; Capo, Tom R et al. (2013) Age-associated bidirectional modulation of gene expression in single identified R15 neuron of Aplysia. BMC Genomics 14:880|
|Akhmedov, Komolitdin; Rizzo, Valerio; Kadakkuzha, Beena M et al. (2013) Decreased response to acetylcholine during aging of aplysia neuron R15. PLoS One 8:e84793|
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