The Viper Resource Center (VRC), P40 competitive renewal, at Texas A&M University-Kingsville proposes to continue the aims of the initial grant application. The resources will be expanded and snake venom, venom fractions, blood and cells will be made available to the biomedical research community. The research segment of the proposal will focus on improving the products by characterizing important molecules found in venom for biomedical research. Because of university support and NIH funding, the VRC within the Natural Toxins Research Center (NTRC) has developed a unique facility with the state-of-the-art instrumentation. There are 300 different species of venomous snakes in the world and venoms are complex mixtures of thousands of molecules. Many venomous snakes use their venoms for capturing prey by interfering with the clotting cascade, which causes the animal to bleed to death. These same molecules could have therapeutic value for preventing heart attacks and strokes. A good example of these molecules is the disintegrins, which are competitive inhibitors of fibrinogen and interfere with platelet function. Disintegrins block cell-to-cell, cell-to-matrix interactions and signal transduction, thereby affecting the internal and external cellular activities of many different cells. It is our hypothesis that snake venoms are abundant and stable sources of many molecules and once purified, characterized, and cloned, could have many potential applications in medicine. Based on this hypothesis, the specific aims are to continue: (1) increasing the number of venomous snakes at the VRC for biomedical use;(2) characterizing snake venoms by high performance liquid chromatography and various assays to determine medical applications of venom molecules;(3) improving the Internet database for better dissemination of information to investigators;and (4) using the latest techniques such as proteomics to determine the molecular weights and amino acid sequences of venom proteins. The expected results will be that investigators will have access to a wide variety of properly maintained snakes, quality venoms and characterized fractions for biomedical research. The snakes, their venoms and venom fractions are placed on the NTRC Internet database, which can be queried. Venom can be ordered at a later date from the same snake, since all snakes have pit tags. Evaluation of the VRC will be based on the number of investigators using the venoms, publications, and information disseminated through the Internet database. The significance of the proposed research is that venom molecules have important biomedical applications in many different areas such as strokes, heart attacks, cancer and other medical problems.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
8P40OD010960-10
Application #
8241954
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Chang, Michael
Project Start
2003-04-15
Project End
2014-04-30
Budget Start
2012-05-01
Budget End
2014-04-30
Support Year
10
Fiscal Year
2012
Total Cost
$489,552
Indirect Cost
$123,221
Name
Texas A&M University-Kingsville
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
868154089
City
Kingsville
State
TX
Country
United States
Zip Code
78363
Dobson, James; Yang, Daryl C; Op den Brouw, Bianca et al. (2018) Rattling the border wall: Pathophysiological implications of functional and proteomic venom variation between Mexican and US subspecies of the desert rattlesnake Crotalus scutulatus. Comp Biochem Physiol C Toxicol Pharmacol 205:62-69
Nielsen, Vance G; Sánchez, Elda E; Redford, Daniel T (2018) Characterization of the Rabbit as an In Vitro and In Vivo Model to Assess the Effects of Fibrinogenolytic Activity of Snake Venom on Coagulation. Basic Clin Pharmacol Toxicol 122:157-164
Schield, Drew R; Adams, Richard H; Card, Daren C et al. (2017) Insight into the roles of selection in speciation from genomic patterns of divergence and introgression in secondary contact in venomous rattlesnakes. Ecol Evol 7:3951-3966
Komives, Claire F; Sanchez, Elda E; Rathore, Anurag S et al. (2017) Opossum peptide that can neutralize rattlesnake venom is expressed in Escherichia coli. Biotechnol Prog 33:81-86
Rokyta, Darin R; Margres, Mark J; Ward, Micaiah J et al. (2017) The genetics of venom ontogeny in the eastern diamondback rattlesnake (Crotalus adamanteus). PeerJ 5:e3249
Zhang, Chuchu; Medzihradszky, Katalin F; Sánchez, Elda E et al. (2017) Lys49 myotoxin from the Brazilian lancehead pit viper elicits pain through regulated ATP release. Proc Natl Acad Sci U S A 114:E2524-E2532
Cantú Jr, Esteban; Mallela, Sahiti; Nyguen, Matthew et al. (2017) The binding effectiveness of anti-r-disintegrin polyclonal antibodies against disintegrins and PII and PIII metalloproteases: An immunological survey of type A, B and A+B venoms from Mohave rattlesnakes. Comp Biochem Physiol C Toxicol Pharmacol 191:168-176
Dowell, Noah L; Giorgianni, Matt W; Kassner, Victoria A et al. (2016) The Deep Origin and Recent Loss of Venom Toxin Genes in Rattlesnakes. Curr Biol 26:2434-2445
Margres, Mark J; Walls, Robert; Suntravat, Montamas et al. (2016) Functional characterizations of venom phenotypes in the eastern diamondback rattlesnake (Crotalus adamanteus) and evidence for expression-driven divergence in toxic activities among populations. Toxicon 119:28-38
Borja, Miguel; Galan, Jacob Anthony; Cantu Jr, Esteban et al. (2016) Morulustatin, A Disintegrin that Inhibits ADP-Induced Platelet Aggregation, Isolated from the Mexican Tamaulipan Rock Rattlesnake (Crotalus lepidus morulus). Revista cientifica (Universidad del Zulia. Facultad de Ciencias 26:86-94

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