The overall goal of this proposal is to continue and expand the functions and capabilities of the Rat Resource and Research Center (RRRC) to provide biomedical investigators with the rat models, ES cells, related reagents and protocols they require for their research. In the last two years the biomedical community has developed several technologies to make targeted mutations (e.g., knock-outs and knock-ins) in rats. As a result the number of genetically engineered rat models and the number of investigators using rat models for biomedical research is likely to increase dramatically. The RRRC provides a unique repository service to the biomedical community for importing, storing and distributing a vast number of rat strains;as well as performing research that improves the function of the Resource Center. In addition to the animal repository, cryostorage, and distribution functions, the RRRC proposes to expand its function to assist investigators in the use of new rat gene targeting technologies and the unique challenges associated with these. The RRRC will do this by providing protocols for targeting and ES cell culture along with technical training in rat gene targeting. By expanding our services, the RRRC will serve as a much-needed resource for biomedical research investigators using rats in biomedical research.

Public Health Relevance

The overall goal of this proposal is to continue and expand the functions and capabilities of the Rat Resource and Research Center (RRRC) to provide biomedical investigators with the rat models, ES cells, related reagents and protocols they require for their research.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
3P40OD011062-14S1
Application #
8869084
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Mirochnitchenko, Oleg
Project Start
2001-06-01
Project End
2016-05-31
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
14
Fiscal Year
2014
Total Cost
$249,998
Indirect Cost
$69,243
Name
University of Missouri-Columbia
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
153890272
City
Columbia
State
MO
Country
United States
Zip Code
65211
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Shimoyama, Mary; Smith, Jennifer R; Bryda, Elizabeth et al. (2017) Rat Genome and Model Resources. ILAR J 58:42-58
Ma, Man Chun John; Pettus, Janette M; Jakoubek, Jessica A et al. (2017) Contribution of independent and pleiotropic genetic effects in the metabolic syndrome in a hypertensive rat. PLoS One 12:e0182650
Mamad, Omar; Stumpp, Lars; McNamara, Harold M et al. (2017) Place field assembly distribution encodes preferred locations. PLoS Biol 15:e2002365
Li, Shuping; Lan, He; Men, Hongsheng et al. (2016) Derivation of Transgene-Free Rat Induced Pluripotent Stem Cells Approximating the Quality of Embryonic Stem Cells. Stem Cells Transl Med :
Hansen, Sarah A; Hart, Marcia L; Busi, Susheel et al. (2016) Fischer-344 Tp53-knockout rats exhibit a high rate of bone and brain neoplasia with frequent metastasis. Dis Model Mech 9:1139-1146
Kim, Suhee; Hooper, Sarah; Agca, Cansu et al. (2016) Post-thaw ATP supplementation enhances cryoprotective effect of iodixanol in rat spermatozoa. Reprod Biol Endocrinol 14:5
Hanson, Marina M; Liu, Fengming; Dai, Shen et al. (2016) Rapid conditional targeted ablation model for hemolytic anemia in the rat. Physiol Genomics 48:626-32
Lan, He; Li, Shuping; Guo, Zihang et al. (2016) Efficient generation of selection-gene-free rat knockout models by homologous recombination in ES cells. FEBS Lett 590:3416-3424
Ericsson, Aaron C; Franklin, Craig L (2015) Manipulating the Gut Microbiota: Methods and Challenges. ILAR J 56:205-17

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