This resubmission of our animal resource grant renewal proposal requests five years of funding to continue support for a Specific Pathogen Free (SPF) colony of pig-tailed macaques (Macaca nemestrina) at the Johns Hopkins University that was first funded on September 21, 2006. It has the following resource component and research component aims: A. Resource Component Related Aims: (1) To expand the size of the current SPF breeding colony to increase the numbers of females for breeding and males available for sale to NIH funded investigators both within Johns Hopkins University and at other research institutions. (2) To maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV). At present all of the current members of the colony have repeatedly tested negative for these agents. (3) To maintain pedigree and health data on all animals. This data is available on all of the animals in the colony and will be expanded as the colony grows. B. Research Component Aims: To expand the genetic data available for this species to a) increase the research utility of the species and b) assist in disease management of the colony. This will be done through three research aims. (1) To characterize pig-tailed macaque immunogenetics in detail via MHC class I genotyping. (2) To characterize pig-tailed macaque genetics by single nucleotide polymorphism (SNP) genetic marker mapping throughout the pig-tailed macaque genome. (3) To determine whether either MHC class I allele expression or SNPs are associated with a) SIV-related disease outcomes and b) naturally occurring enterocolitis in pig-tailed macaques.
Specific pathogen free pig-tailed macaques are in short supply in the US at this time, yet they are important animal models in a variety of disease entities. This colony is one of the two such colonies in the US, and will provide animals to NIH funded investigators. The research aims of this project are important to expanding the knowledge base of this macaque species which will increase its utility as an animal model of human disease.
|Beck, Sarah E; Queen, Suzanne E; Viscidi, Raphael et al. (2016) Central nervous system-specific consequences of simian immunodeficiency virus Gag escape from major histocompatibility complex class I-mediated control. J Neurovirol 22:498-507|
|Avalos, Claudia R; Price, Sarah L; Forsyth, Ellen R et al. (2016) Quantitation of Productively Infected Monocytes and Macrophages of Simian Immunodeficiency Virus-Infected Macaques. J Virol 90:5643-56|
|Buchanan, Erin L; Espinoza, Diego A; McAlexander, Melissa A et al. (2016) SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load. Sci Rep 6:22629|
|Mangus, Lisa M; Dorsey, Jamie L; Weinberg, Rachel L et al. (2016) Tracking Epidermal Nerve Fiber Changes in Asian Macaques: Tools and Techniques for Quantitative Assessment. Toxicol Pathol 44:904-12|
|Williams, Dionna W; Engle, Elizabeth L; Shirk, Erin N et al. (2016) Splenic Damage during SIV Infection: Role of T-Cell Depletion and Macrophage Polarization and Infection. Am J Pathol 186:2068-87|
|Izzi, Jessica M; Beck, Sarah E; Adams, Robert J et al. (2016) Serum Cobalamin (Vitamin B12) Concentrations in Rhesus Macaques (Macaca mulatta) and Pigtailed Macaques (Macaca nemestrina) with Chronic Idiopathic Diarrhea. Comp Med 66:324-32|
|Witwer, Kenneth W; Buchanan, Erin L; Myers, Stephanie L et al. (2015) miRNAs and SAMHD1 regulation in vitro and in a model of HIV CNS disease. J Neuroinflammation 12:159|
|Muth, Dillon C; McAlexander, Melissa A; Ostrenga, Lauren J et al. (2015) Potential role of cervicovaginal extracellular particles in diagnosis of endometriosis. BMC Vet Res 11:187|
|Beck, Sarah E; Queen, Suzanne E; Witwer, Kenneth W et al. (2015) Paving the path to HIV neurotherapy: Predicting SIV CNS disease. Eur J Pharmacol 759:303-12|
|Freeman, Zachary T; Krall, Caroline; Rice, Kelly A et al. (2015) Severity and Distribution of Wounds in Rhesus Macaques (Macaca mulatta) Correlate with Observed Self-Injurious Behavior. J Am Assoc Lab Anim Sci 54:516-20|
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