This is a competing renewal application for a Center to prepare adult stem/progenitor cells from the bone marrow of human volunteers and rodents, define the quality of the cells, and then distribute them to multiple investigators. The adult stem/progenitor cells we prepare were originally referred to as fibroblastic colony forming - units, then as marrow stromal cells in the hematological literature, subsequently as mesenchymal stem cells, and most recently as multipotent mesenchymal stromal cells or MSCs. Since its establishment in June of 2003, the Center has made 451 shipments of 1,013 vials of frozen MSCs to 220 individual investigators, 129 of which are NIH funded (Appendix A). An additional 41 investigators have submitted MTAs to receive MSCs;of which 14 are NIH funded. A total of 114 investigators have provided letters of support for this competing renewal application.
The Specific Aims are: 1. To continue to produce standardized preparations of human MSCs (hMSCs) for distribution to other investigators. 2. To continue to provide similarly characterized preparations of rat MSCs (rMSCs) for distribution to other investigators. 3. To continue to provide similar preparations of mouse MSCs (moMSCs) for distribution to other investigators. 4. To develop improved methods for isolating and characterizing hMSCs. The need for experiments with fully characterized MSCs has become critical with the current efforts by us and others to initiate Phase I and Phase II clinical trials with MSCs.
|Bajcsy, Peter; Yoon, Soweon; Florczyk, Stephen J et al. (2017) Modeling, validation and verification of three-dimensional cell-scaffold contacts from terabyte-sized images. BMC Bioinformatics 18:526|
|Clough, Bret H; McNeill, Eoin P; Palmer, Daniel et al. (2017) An allograft generated from adult stem cells and their secreted products efficiently fuses vertebrae in immunocompromised athymic rats and inhibits local immune responses. Spine J 17:418-430|
|Chen, Honglin; Malheiro, Afonso de Botelho Ferreira Braga; van Blitterswijk, Clemens et al. (2017) Direct Writing Electrospinning of Scaffolds with Multidimensional Fiber Architecture for Hierarchical Tissue Engineering. ACS Appl Mater Interfaces 9:38187-38200|
|Shih, Yu-Ru; Kang, Heemin; Rao, Vikram et al. (2017) In vivo engineering of bone tissues with hematopoietic functions and mixed chimerism. Proc Natl Acad Sci U S A 114:5419-5424|
|Morrison, Thomas J; Jackson, Megan V; Cunningham, Erin K et al. (2017) Mesenchymal Stromal Cells Modulate Macrophages in Clinically Relevant Lung Injury Models by Extracellular Vesicle Mitochondrial Transfer. Am J Respir Crit Care Med 196:1275-1286|
|Whitely, Michael E; Robinson, Jennifer L; Stuebben, Melissa C et al. (2017) Prevention of Oxygen Inhibition of PolyHIPE Radical Polymerization using a Thiol-based Crosslinker. ACS Biomater Sci Eng 3:409-419|
|Clough, Bret H; Ylostalo, Joni; Browder, Elizabeth et al. (2017) Theobromine Upregulates Osteogenesis by Human Mesenchymal Stem Cells In Vitro and Accelerates Bone Development in Rats. Calcif Tissue Int 100:298-310|
|Jackson, Megan V; Krasnodembskaya, Anna D (2017) Analysis of Mitochondrial Transfer in Direct Co-cultures of Human Monocyte-derived Macrophages (MDM) and Mesenchymal Stem Cells (MSC). Bio Protoc 7:|
|Shigemoto-Kuroda, Taeko; Oh, Joo Youn; Kim, Dong-Ki et al. (2017) MSC-derived Extracellular Vesicles Attenuate Immune Responses in Two Autoimmune Murine Models: Type 1 Diabetes and Uveoretinitis. Stem Cell Reports 8:1214-1225|
|Cruz, Fernanda F; Borg, Zachary D; Goodwin, Meagan et al. (2016) CD11b+ and Sca-1+ Cells Exert the Main Beneficial Effects of Systemically Administered Bone Marrow-Derived Mononuclear Cells in a Murine Model of Mixed Th2/Th17 Allergic Airway Inflammation. Stem Cells Transl Med 5:488-99|
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