The Center for Neuroanatomy with Neurotropic Viruses (CNNV) was funded in 2004 to address the increasing needs of the research community for access to the use of viral transneuronal tracing tracers. This approach is the most widely used method to gain a circuit perspective on the functional architecture of the nervous system at a cellular level. The CNNV has become an integral resource for investigators by providing well-characterized reagents, guidance in the use of the method and translational support for successful grant applications. This application for continued funding of the CNNV is founded on the same goals and rationale advanced in the original submission. Specifically we propose to maintain a state-of-the- art National Resource that a) serves as a technical and intellectual resource for those interested in using the method, b) develops improved transneuronal tracing tools that permit more targeted dissection of the functional architecture of neural circuits, c) serves as a repository for well-characterized reagents essential to application of the method, and d) supports and stimulates the research enterprise of the NIH and other research agencies by providing sophisticated tools that enhance and expand the capabilities of individual investigators. The belief that this can best be achieved through the CNNV Is based upon the following assertions. First, by providing well-characterized reagents and essential expertise, the CNNV substantially reduces barriers that investigators experience in seeking access to the use of viruses as transneuronal tracers. Second, the unique combination of multidisciplinary expertise available through the CNNV provides the focus and platform for more rapid, targeted and Informed technology development. Third, the existence of unique resources and facilities within core laboratories of the CNNV greatly expands access to the technology. In this application we provide objective evidence supporting each of these assertions and demonstrate that the CNNV has rapidly evolved into a critical National Resource for the neuroscience research community. We also highlight the accomplishments of the Center and provide a plan for further improving the service and technology functions of the CNNV.
(provided by applicant): The ability to treat disease and injury of the brain depends upon developing a fundamental understanding of the complex neural circuits that comprise the central nervous system. The use of neurotropic viruses as transneuronal tracers provides a unique and powerful tool that reveals the functional organization of these complex circuits. The CNNV is a national resource that provides and develops the reagents for this purpose.
|Ryu, Vitaly; Watts, Alan G; Xue, Bingzhong et al. (2017) Bidirectional crosstalk between the sensory and sympathetic motor systems innervating brown and white adipose tissue in male Siberian hamsters. Am J Physiol Regul Integr Comp Physiol 312:R324-R337|
|Nair, Jayakrishnan; Bezdudnaya, Tatiana; Zholudeva, Lyandysha V et al. (2017) Histological identification of phrenic afferent projections to the spinal cord. Respir Physiol Neurobiol 236:57-68|
|Livneh, Yoav; Ramesh, Rohan N; Burgess, Christian R et al. (2017) Homeostatic circuits selectively gate food cue responses in insular cortex. Nature 546:611-616|
|Anwar, Imran J; Miyata, Kayoko; Zsombok, Andrea (2016) Brain stem as a target site for the metabolic side effects of olanzapine. J Neurophysiol 115:1389-98|
|Rosario, Wilfredo; Singh, Inderroop; Wautlet, Arnaud et al. (2016) The Brain-to-Pancreatic Islet Neuronal Map Reveals Differential Glucose Regulation From Distinct Hypothalamic Regions. Diabetes 65:2711-23|
|Nam, H; Kerman, I A (2016) Distribution of catecholaminergic presympathetic-premotor neurons in the rat lower brainstem. Neuroscience 324:430-45|
|Davis, Benjamin M; Rall, Glenn F; Schnell, Matthias J (2015) Everything You Always Wanted to Know About Rabies Virus (But Were Afraid to Ask). Annu Rev Virol 2:451-71|
|Parsons, Lance R; Tafuri, Yolanda R; Shreve, Jacob T et al. (2015) Rapid genome assembly and comparison decode intrastrain variation in human alphaherpesviruses. MBio 6:|
|Taylor, Matthew P; Enquist, Lynn W (2015) Axonal spread of neuroinvasive viral infections. Trends Microbiol 23:283-8|
|Engel, Esteban A; Song, Ren; Koyuncu, Orkide O et al. (2015) Investigating the biology of alpha herpesviruses with MS-based proteomics. Proteomics 15:1943-56|
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