This proposal requests continued support for the MIT/Harvard Center for Magnetic Resonance (CMR) located at the Francis Bitter Magnet Laboratory, MIT (EB002026). During the coming five-year period we plan significant upgrades of existing equipment, acquisition of new instrumentation, and research initiatives in several new areas. During the proposed five year grant period, we plan to initiate or complete construction or acquisition of the following new pieces of equipment, which will be integrated into the facility. These instruments will include the following: (1) a 460 GHz/700 MHz dynamic nuclear polarization (DNP)/NMR spectrometer;(2) an 800 MHz/89 mm NMR primarily for solids and conversion of the 750/62 to a solution spectrometer;(3) a 140 GHz gyroamplifier for time domain DNP and EPR experiments;(4) a 600 MHz/125 mm spectrometer for temperature jump DNP experiments. This will entail construction of a 395 GHz gyrotron oscillator for the DNP experiments;(5) a helium recirculation system for MAS experiments in the 20-30 K regime;(6) a tunable 330 GHz gyrotron oscillator that will be interfaced to an existing 500/104 spectrometer;(7) a commercial 9 GHz time domain EPR for distance measurements. In addition we will continue the strong core, collaborative and user research programs. Scientific topics include membrane proteins, signaling, metabolomics, and amyloid proteins associated with disease.

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Biotechnology Resource Grants (P41)
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Special Emphasis Panel (ZRG1-BCMB-N (40))
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Sastre, Antonio
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Massachusetts Institute of Technology
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Edmonds, Katherine A; Wagner, Gerhard (2015) (1)H, (13)C, and (15)N backbone and sidechain chemical shift assignments for the HEAT2 domain of human eIF4GI. Biomol NMR Assign 9:157-60
Takeuchi, Koh; Sun, Zhen-Yu J; Li, Shuai et al. (2015) NMR resonance assignments of the catalytic domain of human serine/threonine phosphatase calcineurin in unligated and PVIVIT-peptide-bound states. Biomol NMR Assign 9:201-5
Valentine, Kathleen G; Mathies, Guinevere; Bédard, Sabrina et al. (2014) Reverse micelles as a platform for dynamic nuclear polarization in solution NMR of proteins. J Am Chem Soc 136:2800-7
Ravera, Enrico; Corzilius, Björn; Michaelis, Vladimir K et al. (2014) DNP-enhanced MAS NMR of bovine serum albumin sediments and solutions. J Phys Chem B 118:2957-65
Brazin, Kristine N; Mallis, Robert J; Li, Chen et al. (2014) Constitutively oxidized CXXC motifs within the CD3 heterodimeric ectodomains of the T cell receptor complex enforce the conformation of juxtaposed segments. J Biol Chem 289:18880-92
Can, T V; Caporini, M A; Mentink-Vigier, F et al. (2014) Overhauser effects in insulating solids. J Chem Phys 141:064202
Akabayov, Sabine R; Wagner, Gerhard (2014) Backbone resonance assignment of the HEAT1-domain of the human eukaryotic translation initiation factor 4GI. Biomol NMR Assign 8:89-91
Bayro, Marvin J; Chen, Bo; Yau, Wai-Ming et al. (2014) Site-specific structural variations accompanying tubular assembly of the HIV-1 capsid protein. J Mol Biol 426:1109-27
Su, Yongchao; Sarell, Claire J; Eddy, Matthew T et al. (2014) Secondary structure in the core of amyloid fibrils formed from human ??m and its truncated variant ?N6. J Am Chem Soc 136:6313-25
Kiesewetter, Matthew K; Michaelis, Vladimir K; Walish, Joseph J et al. (2014) High field dynamic nuclear polarization NMR with surfactant sheltered biradicals. J Phys Chem B 118:1825-30

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