This proposal requests continued support for the MIT/Harvard Center for Magnetic Resonance (CMR) located at the Francis Bitter Magnet Laboratory, MIT, a joint effort between MIT and Harvard Medical School. During the coming five-year period we plan significant upgrades of existing equipment, acquisition of new instrumentation, and research initiatives in several new areas. Specifically, the P41 grant will continue to support an exciting core research program in magnetic resonance based structural biology focused on studies of membrane proteins, translation initiation, amyloid, new high frequency microwave technology for magnetic, methods for non-uniform sampling and dynamic nuclear polarization, and high temperature superconducting magnets for NMR. In addition, we will be developing new instrumentation as follows: (1) T-locks for the solution 750 and 900 MHz spectrometers; (2) upgrading of a 460 GHz/700 MHz dynamic nuclear polarization (DNP)/NMR spectrometer; (3) design and construction of helium recirculation system for low temperature DNP experiments in the 10-60 K regime; (4) design and construction of a new generation of MAS probes that are balanced on all channels; (5) design and construction of the next generation of NMR console on a chip. The research of the five TR&D's will implement new technology for NUS, for preparation and analysis of membrane protein structures, studies of sedimented samples, high frequency MAS for membrane and amyloid protein, improving resolution at low temperatures and 17-O labeling and spectroscopy.

Public Health Relevance

The CMR consists of a cluster of high field NMR and EPR spectrometers devoted to long term experiments in structural biology. In addition, many new exciting applications are being pursued particularly in the area of membrane protein and amyloid fibril structure. The CMR is heavily involved in collaborations and is open to the user community interested in high field magnetic resonance.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Biotechnology Resource Grants (P41)
Project #
2P41EB002026-39
Application #
8667750
Study Section
Special Emphasis Panel (ZEB1-OSR-E (J2))
Program Officer
Sastre, Antonio
Project Start
1976-05-30
Project End
2019-04-30
Budget Start
2014-05-30
Budget End
2015-04-30
Support Year
39
Fiscal Year
2014
Total Cost
$1,314,750
Indirect Cost
$362,509
Name
Massachusetts Institute of Technology
Department
None
Type
Organized Research Units
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Schaub, S C; Shapiro, M A; Temkin, R J (2016) Simple Expressions for the Design of Linear Tapers in Overmoded Corrugated Waveguides. J Infrared Millim Terahertz Waves 37:100-110
Sun, Zhen-Yu J; Bhanu, Meera K; Allan, Martin G et al. (2016) Solution Structure of the Cuz1 AN1 Zinc Finger Domain: An Exposed LDFLP Motif Defines a Subfamily of AN1 Proteins. PLoS One 11:e0163660
Takeuchi, Koh; Arthanari, Haribabu; Imai, Misaki et al. (2016) Nitrogen-detected TROSY yields comparable sensitivity to proton-detected TROSY for non-deuterated, large proteins under physiological salt conditions. J Biomol NMR 64:143-51
Zhukhovitskiy, Aleksandr V; Zhong, Mingjiang; Keeler, Eric G et al. (2016) Highly branched and loop-rich gels via formation of metal-organic cages linked by polymers. Nat Chem 8:33-41
Dev, Jyoti; Park, Donghyun; Fu, Qingshan et al. (2016) Structural basis for membrane anchoring of HIV-1 envelope spike. Science 353:172-5
Viegas, Aldino; Viennet, Thibault; Yu, Tsyr-Yan et al. (2016) UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection. J Biomol NMR 64:9-15
Fu, Qingshan; Fu, Tian-Min; Cruz, Anthony C et al. (2016) Structural Basis and Functional Role of Intramembrane Trimerization of the Fas/CD95 Death Receptor. Mol Cell 61:602-13
Salvi, Nicola; Papadopoulos, Evangelos; Blackledge, Martin et al. (2016) The Role of Dynamics and Allostery in the Inhibition of the eIF4E/eIF4G Translation Initiation Factor Complex. Angew Chem Int Ed Engl 55:7176-9
Nishikawa, Joy L; Boeszoermenyi, Andras; Vale-Silva, Luis A et al. (2016) Inhibiting fungal multidrug resistance by disrupting an activator-Mediator interaction. Nature 530:485-9
Oxenoid, Kirill; Dong, Ying; Cao, Chan et al. (2016) Architecture of the mitochondrial calcium uniporter. Nature 533:269-73

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