The fundamental goal of the Driving Biomedical Projects (DBPs) portfolio is to provide synergism, motivation, and act as a catalyst that drives the Washington University (WU) Biomedical Mass Spectrometry (MS) Resource personnel to develop and advance novel MS technologies, techniques, and methods (i.e., TRDs) that overcome technical challenges that inhibit progress on critical biomedical research questions or the biomolecular basis for a significant clinical disorder. Our DBPs provide a challenging biological platform or context upon which we can test, develop, refine, enhance, and advance new MS technologies and techniques. DBPs give purpose and compel Resource personnel to brainstorm, develop, custom-design, test, and apply MS technology and techniques in novel and innovative directions. When successful, they provide for the biomedical research community new tools that can be applied to solve intractable analytical problems. Our DBP portfolio has been carefully integrated with our proposed TRD themes, and includes 7 new, 29 ongoing, and 30 DBPs completed from 2009-13.
-Public Health Relevance. The Washington University Biomedical Mass Spectrometry Resource has a longstanding history as an active and productive citizen in the NIH Biotechnology Research Resources community. We propose to extend our mission by advancing mass spectrometry technology, development, and research, applying these discoveries to answer critical biomedical research questions, and training the next generation of researchers, towards the ultimate goal of improving public health.
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|Domingo-Gonzalez, Racquel; Das, Shibali; Griffiths, Kristin L et al. (2017) Interleukin-17 limits hypoxia-inducible factor 1? and development of hypoxic granulomas during tuberculosis. JCI Insight 2:|
|Frieden, Carl; Wang, Hanliu; Ho, Chris M W (2017) A mechanism for lipid binding to apoE and the role of intrinsically disordered regions coupled to domain-domain interactions. Proc Natl Acad Sci U S A 114:6292-6297|
|Reeds, Dominic N; Pietka, Terri A; Yarasheski, Kevin E et al. (2017) HIV infection does not prevent the metabolic benefits of diet-induced weight loss in women with obesity. Obesity (Silver Spring) 25:682-688|
|Wang, Hanliu; Shu, Qin; Frieden, Carl et al. (2017) Deamidation Slows Curli Amyloid-Protein Aggregation. Biochemistry 56:2865-2872|
|Lu, Yue; Liu, Haijun; Saer, Rafael G et al. (2017) Native Mass Spectrometry Analysis of Oligomerization States of Fluorescence Recovery Protein and Orange Carotenoid Protein: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle. Biochemistry 56:160-166|
|Zhang, Hao; Harrington, Lucas B; Lu, Yue et al. (2017) Native Mass Spectrometry Characterizes the Photosynthetic Reaction Center Complex from the Purple Bacterium Rhodobacter sphaeroides. J Am Soc Mass Spectrom 28:87-95|
|Ohlemacher, Shannon I; Giblin, Daryl E; d'Avignon, D André et al. (2017) Enterobacteria secrete an inhibitor of Pseudomonas virulence during clinical bacteriuria. J Clin Invest 127:4018-4030|
|Niu, Ben; Mackness, Brian C; Rempel, Don L et al. (2017) Incorporation of a Reporter Peptide in FPOP Compensates for Adventitious Scavengers and Permits Time-Dependent Measurements. J Am Soc Mass Spectrom 28:389-392|
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