This proposal is a competing renewal for the NIGMS P41 National Center for Biomedical Glycomics. Glycomics can be defined in several ways, but in terms of our Center, we define Glycomics as the study of glycans, glycoconjugates, the proteins that regulate their expression, and the proteins that recognize them;in particular, we are interested in developing tools to facilitate learning how these molecules change during early embryonic development and during the onset of disease as a result of mutations that affect glycan expression. To develop technologies that will impact the understanding and regulation of the diversity of glycosylation, therefore, we felt it necessary to focus on Glycomics from more of a Systems Biology approach: investigation of glycosylation in a particular biological context, analysis of the fine details of glycan expression on glycoconjugates produced in specific cell types, identification of the glyco-genes that participate in glycan biosynthesis and the biosynthetic pathways that the enzymes and transporters expressed from the glyco-genes constitute. The structure of the Resource is focused on four Technological Research &Development programs plus Analytical Service and Training: TR&D1: Stem Cell and Induced Pluripotent Stem Cell (IPSC) Resources;TR&D2: Glycomics and Glycoproteomics;TR&D3: Transcriptome and Glycome Regulation;TR&D4: Glycobioinformatics. Our Center lists 31 DBPs, 73 Collaborative Projects, 192 Service projects, and four Courses/Workshops that have had 245 participants over the last four years. We also report 83 publications resulting from research associated with the Center. We have developed technologies in each of our TR&D that have significantly impacted the biomedical research community, and our over-arching goal is to amplify that impact over the next funding period.

Public Health Relevance

The study of Glycomics is in its early stages and is very dependent on the development of tools and technologies. Glycomics involves types of molecules called glycans that are on the surfaces of cells which regulate many physiological processes such as inflammation and innate immunity. We have developed tools to assist biomedical researchers in understanding the structure and functions of these fascinating biomolecules.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
5P41GM103490-12
Application #
8708154
Study Section
Special Emphasis Panel (ZRG1-IMST-J (40))
Program Officer
Sheeley, Douglas
Project Start
2003-09-01
Project End
2018-05-31
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
12
Fiscal Year
2014
Total Cost
$1,704,118
Indirect Cost
$561,054
Name
University of Georgia
Department
Type
Organized Research Units
DUNS #
004315578
City
Athens
State
GA
Country
United States
Zip Code
30602
Itoh, Kazuyoshi; Akimoto, Yoshihiro; Kondo, Shu et al. (2018) Glucuronylated core 1 glycans are required for precise localization of neuromuscular junctions and normal formation of basement membranes on Drosophila muscles. Dev Biol 436:108-124
Tanigaki, Keiji; Sacharidou, Anastasia; Peng, Jun et al. (2018) Hyposialylated IgG activates endothelial IgG receptor Fc?RIIB to promote obesity-induced insulin resistance. J Clin Invest 128:309-322
Kudelka, Matthew R; Nairn, Alison V; Sardar, Mohammed Y et al. (2018) Isotopic labeling with cellular O-glycome reporter/amplification (ICORA) for comparative O-glycomics of cultured cells. Glycobiology 28:214-222
Gonzalez-Gil, Anabel; Porell, Ryan N; Fernandes, Steve M et al. (2018) Sialylated keratan sulfate proteoglycans are Siglec-8 ligands in human airways. Glycobiology 28:786-801
Williams, Caitlin R; Chen, Li; Driver, Ashley D et al. (2018) Sialylated Receptor Setting Influences Mycoplasma pneumoniae Attachment and Gliding Motility. Mol Microbiol 109:735-744
Miller, James J; Aoki, Kazuhiro; Moehring, Francie et al. (2018) Neuropathic pain in a Fabry disease rat model. JCI Insight 3:
Carroll, Daniela J; O'Sullivan, Jeremy A; Nix, David B et al. (2018) Sialic acid-binding immunoglobulin-like lectin 8 (Siglec-8) is an activating receptor mediating ?2-integrin-dependent function in human eosinophils. J Allergy Clin Immunol 141:2196-2207
Middleton, Dustin R; Zhang, Xing; Wantuch, Paeton L et al. (2018) Identification and characterization of the Streptococcus pneumoniae type 3 capsule-specific glycoside hydrolase of Paenibacillus species 32352. Glycobiology 28:90-99
Selvan, Nithya; George, Stephan; Serajee, Fatema J et al. (2018) O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signaling. J Biol Chem 293:10810-10824
King, Samuel R; Hecht, Elizabeth S; Muddiman, David C (2018) Demonstration of hydrazide tagging for O-glycans and a central composite design of experiments optimization using the INLIGHT™ reagent. Anal Bioanal Chem 410:1409-1415

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