The ability to precisely and accurately measure levels of nearly all expressed proteins and their modified forms can provide new insights into the molecular nature and operation of cells, cell signaling pathways and networks, the cell cycle, cellular differentiation, and other processes relevant to human health and to the progression of various disease states. This ?4i Resource Center is focused on serving the NIH-supported biomedical research community by developing and integrating new proteomic technologies for biological applications, disseminating the new technologies, and training scientists in their use. In its first 4 years the Resource Center has cultivated a strong team of researchers, who have achieved a strong publication record, a productive set of collaborative projects, and provided effective dissemination of an array of technology and informatics developments. As a result the Center is now poised for even greater successes. The primary technology objectives of the Resource Center Cores are to develop and apply an integrated set of biological methods, new analytical technologies, and associated computational and informatics tools, for much more rapid, quantitative, sensitive, and comprehensive proteomics measurement applications than presently possible. The three technological Cores of the Resource Center aim at development of: i) improved technologies for spatial and temporal proteomics, which includes approaches to better define the compartment localization of proteins, primarily with respect to membrane compartments and to quantify the dynamics of proteins between compartments;2) higher throughput, more sensitive, and much more robust quantitative measurements of proteomes that provide broader dynamic range and greater coverage of proteins and their modifications;and 3) computational and bioinformatics tools needed to provide information based on data with statistically sound measures of quality, so as to facilitate new biological understandings. The Center's technology developments will be evaluated in the context of a range of challenging collaborative applications selected on the basis of their scientific and biomedical relevance, as well as their capacity to benefit from the Resource Center's capabilities. The scope of applications include areas of microbial pathogenicity, trauma research, cellular migration, kidney transplantation, signal transduction, neuroproteomics, radiation response, chronic virulent infections, biomarker discovery, and mammary oncology.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BCMB-H (40))
Program Officer
Sheeley, Douglas
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Battelle Pacific Northwest Laboratories
United States
Zip Code
Na, Seungjin; Payne, Samuel H; Bandeira, Nuno (2016) Multi-species Identification of Polymorphic Peptide Variants via Propagation in Spectral Networks. Mol Cell Proteomics 15:3501-3512
Chen, Tsung-Chi; Ibrahim, Yehia M; Webb, Ian K et al. (2016) Mobility-Selected Ion Trapping and Enrichment Using Structures for Lossless Ion Manipulations. Anal Chem 88:1728-33
Wang, Hui; Shi, Tujin; Qian, Wei-Jun et al. (2016) The clinical impact of recent advances in LC-MS for cancer biomarker discovery and verification. Expert Rev Proteomics 13:99-114
Park, Chung-Min; Johnson, Brett A; Duan, Jicheng et al. (2016) 9-Fluorenylmethyl (Fm) Disulfides: Biomimetic Precursors for Persulfides. Org Lett 18:904-7
Sontag, Ryan L; Nakayasu, Ernesto S; Brown, Roslyn N et al. (2016) Identification of Novel Host Interactors of Effectors Secreted by Salmonella and Citrobacter. mSystems 1:
Nielson, Carrie M; Jones, Kerry S; Chun, Rene F et al. (2016) Free 25-Hydroxyvitamin D: Impact of Vitamin D Binding Protein Assays on Racial-Genotypic Associations. J Clin Endocrinol Metab 101:2226-34
Sadler, Natalie C; Nandhikonda, Premchendar; Webb-Robertson, Bobbie-Jo et al. (2016) Hepatic Cytochrome P450 Activity, Abundance, and Expression Throughout Human Development. Drug Metab Dispos 44:984-91
Perez, Dale M; Richards, Mark P; Parker, Robert S et al. (2016) Role of Cytochrome P450 Hydroxylase in the Decreased Accumulation of Vitamin E in Muscle from Turkeys Compared to that from Chickens. J Agric Food Chem 64:671-80
Gritsenko, Marina A; Xu, Zhe; Liu, Tao et al. (2016) Large-Scale and Deep Quantitative Proteome Profiling Using Isobaric Labeling Coupled with Two-Dimensional LC-MS/MS. Methods Mol Biol 1410:237-47
Chan, Chi Yuet X'avia; Gritsenko, Marina A; Smith, Richard D et al. (2016) The current state of the art of quantitative phosphoproteomics and its applications to diabetes research. Expert Rev Proteomics 13:421-33

Showing the most recent 10 out of 161 publications