Since its incepfion the Yeast Resource Center has specialized in extracting information about protein function from genome sequence. We began when the first eukaryotic genome was completed (budding yeast) and developed an array of technologies to decipher protein funcfion from genome sequence. In this application, we make the natural progression towards understanding how protein variafion affects protein levels, modification, function and structure. We propose to develop technologies in four areas: 1) To identify different protein variants or isoforms. 2) To quantify proteins by mass spectrometry with a focus on determining absolute levels of a protein. 3) To study how protein variation affects protein funcfion. 4) To determine the structures of protein complexes not amenable to high-resolution methods such as x-ray crystallography and NMR.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
8P41GM103533-17
Application #
8320055
Study Section
Special Emphasis Panel (ZRG1-CB-L (40))
Program Officer
Sheeley, Douglas
Project Start
1997-09-30
Project End
2016-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
17
Fiscal Year
2012
Total Cost
$2,078,690
Indirect Cost
$560,078
Name
University of Washington
Department
Biochemistry
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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