Since its inception the Yeast Resource Center has specialized in extracting information about protein function from genome sequence. We began when the first eukaryotic genome was completed (budding yeast) and developed an array of technologies to decipher protein function from genome sequence. In this application, we make the natural progression towards understanding how protein variation affects protein levels, modification, function and structure. We propose to develop technologies in four areas: 1) To identify different protein variants or isoforms. 2) To quantify proteins by mass spectrometry with a focus on determining absolute levels of a protein. 3) To study how protein variation affects protein function. 4) To determine the structures of protein complexes not amenable to high-resolution methods such as x-ray crystallography and NMR.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
5P41GM103533-18
Application #
8508963
Study Section
Special Emphasis Panel (ZRG1-CB-L (40))
Program Officer
Sheeley, Douglas
Project Start
1997-09-30
Project End
2016-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
18
Fiscal Year
2013
Total Cost
$2,373,007
Indirect Cost
$673,288
Name
University of Washington
Department
Biochemistry
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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