Since its inception, the Yeast Resource Center has focused on understanding how genome sequence relates to protein function. The Center began just as budding yeast became the first eukaryote to have its genome sequence completed, and we proposed to use an array of technologies to interpret this sequence. In this application, we undertake novel challenges, centered on understanding how variation in proteins affects their levels, modification, function and structure. We will develop new technologies in three areas: 1) Perturbing and sensing changes to complex pathways; 2) Protein detection and quantitation by mass spectrometry; and 3) Higher order protein structure. These technologies will be driven by ten closely integrated Driving Biomedical Projects. Yeast remains an unparalleled experimental system to develop, test and refine these technologies. As the technologies mature, we will extend their application to higher eukaryotes through internal projects and external collaborations. Furthermore, we make our technologies available as they become robust, through broad collaboration and a well-established track record of dissemination and training.

Public Health Relevance

Our resource is focused on the development of technologies to be used for improving our understanding how genome sequence relates to protein function. This research has played a critical role in the understanding of protein and cellular function. While developed and validated in yeast, these methods impact all aspects of biology.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Sheeley, Douglas
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Washington
Schools of Medicine
United States
Zip Code
Conkar, Deniz; Culfa, Efraim; Odabasi, Ezgi et al. (2017) The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking. J Cell Sci 130:1450-1462
Widjaja, Christella E; Olvera, Jocelyn G; Metz, Patrick J et al. (2017) Proteasome activity regulates CD8+ T lymphocyte metabolism and fate specification. J Clin Invest 127:3609-3623
Hope, Elyse A; Amorosi, Clara J; Miller, Aaron W et al. (2017) Experimental Evolution Reveals Favored Adaptive Routes to Cell Aggregation in Yeast. Genetics 206:1153-1167
Callens, Céline; Coelho, Nelson C; Miller, Aaron W et al. (2017) A multiplex culture system for the long-term growth of fission yeast cells. Yeast 34:343-355
May, Damon H; Tamura, Kaipo; Noble, William S (2017) Param-Medic: A Tool for Improving MS/MS Database Search Yield by Optimizing Parameter Settings. J Proteome Res 16:1817-1824
Hanna 4th, Michael G; Block, Samuel; Frankel, E B et al. (2017) TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER-Golgi intermediate compartments. Proc Natl Acad Sci U S A 114:E7707-E7716
Subramanian, Kanagaraj; Rauniyar, Navin; Lavalleé-Adam, Mathieu et al. (2017) Quantitative Analysis of the Proteome Response to the Histone Deacetylase Inhibitor (HDACi) Vorinostat in Niemann-Pick Type C1 disease. Mol Cell Proteomics 16:1938-1957
Zimmerman, Sandra G; Merrihew, Gennifer E; MacCoss, Michael J et al. (2017) Proteomics Analysis Identifies Orthologs of Human Chitinase-Like Proteins as Inducers of Tube Morphogenesis Defects in Drosophila melanogaster. Genetics 206:973-984
Ma, Yuanhui; McClatchy, Daniel B; Barkallah, Salim et al. (2017) HILAQ: A Novel Strategy for Newly Synthesized Protein Quantification. J Proteome Res 16:2213-2220
Cao, Liwei; Diedrich, Jolene K; Kulp, Daniel W et al. (2017) Global site-specific N-glycosylation analysis of HIV envelope glycoprotein. Nat Commun 8:14954

Showing the most recent 10 out of 328 publications