This proposal seeks to continue and expand technological biomedical research, collaborative, service, training and dissemination activities ofthe Laboratory for Fluorescence Dynamics (LFD). Since 1986, the LFD has been a national resource center dedicated to 1) develop of new fluorescence technologies for biology and medicine and 2) service, training, and dissemination of fluorescence methods in a user facility. In previous years (1986-2010), we established our reputation as the leaders in technological development for fluorescence dynamics. Since moving to UCI in 2006, we expanded the core research and collaborative work in this new fertile biomedical research environment. We created stmctures for training through workshops, tutorials and organization of LFD training courses at UCI and other US universities. Core development expands current limits of biomedically relevant fluorescence instrumentation, particularly for the study of cellular processes. Projects include: 1) new concepts in fluorescence microscopy that develop novel imaging methods to exploit the dynamic fluorescence methodologies that are the hallmark ofthe LFD developments, 2) expanding the concept of spatio-temporal fluctuation correlation analysis to a new powerful image approach that provides detailed information of molecular flow at the nanoscale, 3) new methods for 3D nanoimaging based on the orbital tracking approach, 4) novel technologies for fluorescence lifetime imaging analysis that allow new contrast mechanisms to be applied to tissues and animals, and 5) development of software/algorithm platform for disseminating the methods for data collection and analysis developed at the LFD. Users atthe LFD find ready access to new and innovative technology, which affords them a unique opportunity to rapidly advance their own research programs. Driving biological problems exploit and push technological advances and research in biological processes, macromolecular assembly, tissue organization and dynamics, membrane morphology/function relationships, and innovative biomedical instrumentation. Educational programs include hands-on training of students, postdoctoral fellows and visiting scientists, workshops and specialized schools in innovative fluorescence methodologies.

Public Health Relevance

The proposed technological developments provide real time detection and localization of molecular interactions in 3D in live cells and tissues. We have obtained technological breakthroughs in the areas of microscopy imaging, algorithm developments for revealing comprehensive spatio-temporal correlations, nanoimaging methods with unprecedented resolution and new spectroscopic contrast methods that can identify metabolic states of cells and predict their behavior.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
8P41GM103540-27
Application #
8322671
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Program Officer
Friedman, Fred K
Project Start
1997-08-01
Project End
2016-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
27
Fiscal Year
2012
Total Cost
$1,582,662
Indirect Cost
$401,332
Name
University of California Irvine
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Bhattacharjee, Arunima; Datta, Rupsa; Gratton, Enrico et al. (2017) Metabolic fingerprinting of bacteria by fluorescence lifetime imaging microscopy. Sci Rep 7:3743
Liu, Linan; Zhang, Shirley X; Liao, Wenbin et al. (2017) Mechanoresponsive stem cells to target cancer metastases through biophysical cues. Sci Transl Med 9:
Digiacomo, L; Cardarelli, F; Pozzi, D et al. (2017) An apolipoprotein-enriched biomolecular corona switches the cellular uptake mechanism and trafficking pathway of lipid nanoparticles. Nanoscale 9:17254-17262
Jones, Krysten A; Porterfield, William B; Rathbun, Colin M et al. (2017) Orthogonal Luciferase-Luciferin Pairs for Bioluminescence Imaging. J Am Chem Soc 139:2351-2358
Malacrida, Leonel; Jameson, David M; Gratton, Enrico (2017) A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes. Sci Rep 7:9215
Romero-López, Mónica; Trinh, Andrew L; Sobrino, Agua et al. (2017) Recapitulating the human tumor microenvironment: Colon tumor-derived extracellular matrix promotes angiogenesis and tumor cell growth. Biomaterials 116:118-129
Ceccarelli, Arianna; Di Venere, Almerinda; Nicolai, Eleonora et al. (2017) New insight into the interaction of TRAF2 C-terminal domain with lipid raft microdomains. Biochim Biophys Acta 1862:813-822
Stortz, Martin; Presman, Diego M; Bruno, Luciana et al. (2017) Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape. Sci Rep 7:6219
Herrington, Kari A; Trinh, Andrew L; Dang, Carolyn et al. (2017) Spatial analysis of Cdc42 activity reveals a role for plasma membrane-associated Cdc42 in centrosome regulation. Mol Biol Cell 28:2135-2145
Alfonso-García, Alba; Smith, Tim D; Datta, Rupsa et al. (2016) Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy. J Biomed Opt 21:46005

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