The BioCAT Biotechnology Research Resource operates X-ray beamline 181D at the Advanced Photon Source, Argonne National Laboratory. Now in its 16th year of operation, it is a mature, productive facility with many capabilities unique in the USA, and, arguably, the world. Going forward, we intend to maintain our world-class capabilities in static and time resolved fiber diffraction and small-angle scattering (SAXS) from macromolecules in solution with new emphases on extending SAXS to faster time domains that use less material, and fiber diffraction to smaller spatial domains with wider options on beam sizes, Q ranges and divergences. The BioCAT X-ray florescence microprobe will be made even more efficient and integrated with micro-diffraction mapping, phase contrast and (light) florescence imaging for experiments with substantially more information content. Combining techniques have the potential to greatly accelerate the progress of these types of investigations by making a serial process parallel. A central thread of all core projects will be integrating them with the GDA beamline control environment, developed at the DIAMOND Light Source and adapted here to 18 ID, which will provide a common interface to all experiments and provide advanced support for time resolved experiments. It provides the NeXus file system for efficient handling of very large multidimensional data sets as well as facilitating data archiving and future data mining. Efforts to mitigate radiation damage and a new modeling effort will enhance the validity and information content of muscle diffraction studies. Our Driving Biomedical Projects and collaborative and service projects have relevance to basic mechanisms of muscle function, protein and RNA folding, heart disease, cancer and neurodegenerative diseases. Our proposed training activities are designed to provide users with information and """"""""virtual experiences"""""""" so that they are prepared for their visits and make effective use of the resource. Our proposed dissemination activities are designed to ensure that the scientific community is well-informed of resource capabilities.

Public Health Relevance

The Biophysics Collaborative Access Team uses intense X-ray beams from the Advanced Photon Source, Argonne National Laboratory to perform basic biomedical research on non-crystalline biological materials. Results of this research are expected to be relevant to heart disease, arthritis, cancer, and protein misfolding diseases such as Alzheimer's and ALS.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BCMB-N (40))
Program Officer
Wu, Mary Ann
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Illinois Institute of Technology
Other Basic Sciences
Schools of Arts and Sciences
United States
Zip Code
Forlani, Giuseppe; Nocek, Boguslaw; Chakravarthy, Srinivas et al. (2017) Functional Characterization of Four Putative ?1-Pyrroline-5-Carboxylate Reductases from Bacillus subtilis. Front Microbiol 8:1442
Gates, Zachary P; Baxa, Michael C; Yu, Wookyung et al. (2017) Perplexing cooperative folding and stability of a low-sequence complexity, polyproline 2 protein lacking a hydrophobic core. Proc Natl Acad Sci U S A 114:2241-2246
Fang, Xuan; Liang, Pingdong; Raba, Daniel Alexander et al. (2017) Kinetic characterization of Vibrio cholerae ApbE: Substrate specificity and regulatory mechanisms. PLoS One 12:e0186805
Chen, Yujie; Tokuda, Joshua M; Topping, Traci et al. (2017) Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core. Proc Natl Acad Sci U S A 114:334-339
Ait Mou, Younss; Lacampagne, Alain; Irving, Thomas et al. (2017) Altered myofilament structure and function in dogs with Duchenne muscular dystrophy cardiomyopathy. J Mol Cell Cardiol 114:345-353
Hill, Shannon E; Nguyen, Elaine; Donegan, Rebecca K et al. (2017) Structure and Misfolding of the Flexible Tripartite Coiled-Coil Domain of Glaucoma-Associated Myocilin. Structure 25:1697-1707.e5
Schnicker, Nicholas J; Razzaghi, Mortezaali; Guha Thakurta, Sanjukta et al. (2017) Bacillus anthracis Prolyl 4-Hydroxylase Interacts with and Modifies Elongation Factor Tu. Biochemistry 56:5771-5785
Riback, Joshua A; Bowman, Micayla A; Zmyslowski, Adam M et al. (2017) Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water. Science 358:238-241
Chassé, Maggie H; Muthurajan, Uma M; Clark, Nicholas J et al. (2017) Biochemical and Biophysical Methods for Analysis of Poly(ADP-Ribose) Polymerase 1 and Its Interactions with Chromatin. Methods Mol Biol 1608:231-253
Li, Yue; Zhao, Yuting; Su, Minfei et al. (2017) Structural insights into the interaction of the conserved mammalian proteins GAPR-1 and Beclin 1, a key autophagy protein. Acta Crystallogr D Struct Biol 73:775-792

Showing the most recent 10 out of 109 publications