The BioCAT Biotechnology Research Resource operates X-ray beamline 18ID at the Advanced Photon Source, Argonne National Laboratory. Now in its 20th year of operation, it is a mature, productive facility with many capabilities uniqu in the USA, and, arguably, the world. Going forward, we intend to maintain our world-class capabilities in static, time- and spatially-resolved fiber diffraction with beamline enhancements for increased flux and beam quality. A novel high speed, high sensitivity, high spatial resolution pixel array detector will provide an excellent match to the needs of our muscle diffraction program. Also proposed is a versatile micro-diffraction/micro-SAXS instrument that can use one of two Compound Refractive Lenses optimized for either wide- or small-angle fiber crystallography, and continuous flow SAXS experiments. We will implement multimodal scanning micro-diffraction, x-ray florescence microscopy, phase contrast and uv/visible imaging that can be done either singly or in combination with the same instrument on the same samples. Developments in time-resolved SAXS will extend available time regimes from 500 ns to seconds with major reductions in sample consumption, by more than order of magnitude, from current capabilities. This will allow a much wider range of biomedical problems to be addressed than previously possible. A new beamline data acquisition and control system will provide a common interface and better data management for all experiments and advanced support for time resolved experiments. Combined refractive index, dynamic light scattering and multi-angle light scattering measurements with SAXS will offer more comprehensive sample characterization on-line for more robust results. A multi-scale modeling effort will allow extracting more information from muscle X-ray diffraction studies. The proposed developments in multi-scale simulations for interpreting single molecule SAXS data will profoundly benefit studies of

Public Health Relevance

TheBiophysicsCollaborativeAccessTeamusesintenseX-?raybeamsfromthe AdvancedPhotonSource,ArgonneNationalLaboratorytoperformbasicbiomedical researchonnon-?crystallinebiologicalmaterials.Resultsofthisresearchare expectedtoberelevanttoheartdisease,arthritis,cancer,andproteinmisfolding diseasessuchasAlzheimer'sandALS.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
5P41GM103622-22
Application #
9405015
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Wu, Mary Ann
Project Start
1997-09-30
Project End
2020-12-31
Budget Start
2018-01-01
Budget End
2018-12-31
Support Year
22
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Illinois Institute of Technology
Department
Other Basic Sciences
Type
Schools of Arts and Sciences
DUNS #
042084434
City
Chicago
State
IL
Country
United States
Zip Code
60616
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