Here we propose to establish a """"""""National Center for Functional Glycomics"""""""" (NCFG) that is focused on 5 major objectives;Technology Research and Development (TR&D);Driving Biomedical Projects (DBPs) tied to TR&D Projects;Collaborative Services;Training;and Dissemination of Technologies and Research Discoveries. The development of this application is an outgrowth of the very successful leadership provided by the Glycomics Center at Emory University School of Medicine, which housed the Protein-Glycan Interaction Core (Core H) of the successful Consortium for Functional Glycomics (CFG), a unique NIGMS-funded resource from 2001-2011 that has been utilized by hundreds of investigators worldwide. The Center's applications of the CFG's Defined Glycan Microarray, which has received >800 requests for analyses in the past 8 years, has revolutionized studies on protein-glycan interactions. Defined glycan microarrays have become the benchmark in studying the glycan-binding specificity of lectins, antibodies, and viruses, which for this grant application are termed glycan-binding proteins (GBPs). The value of immobilized glycans in studying GBPs and functional glycomics is evidenced by the collaborations already established in the Center with over 600 Principal Investigators worldwide, resulting in >350 peer-reviewed publications in the past 8 years. Over the past several years, the members of the Glycomics Center at Emory, who are the lead investigators in this proposed NCFG, have provided innovative leadership in developing technologies for studying the functions of cellular glycomes and for glycomic analyses. In the 3 TR&D Projects proposed for this NCFG, we will 1) expand glycan microarray technologies, 2) develop """"""""Shotgun Glycomics"""""""" as a general method for studying natural cell-derived glycan recognition, and 3) develop different glycan display technologies, as well as cellular avatars - particles that represent cellular glycomes in unique presentations. The DBPs include studies on the roles of glycans in microbial and viral infections, glycans and GBPs in cell adhesion, innate and adaptive immune responses, and recognition factors within the glycocalyx that contribute to GBP interactions. The NCFG will use these advances to drive a strong Collaborative Service component to aid the biomedical research community in the using and developing of glycan microarrays and other approaches to explore glycan recognition. We will offer a robust Training program, as well as initiate multiple venues and strategies to Disseminate the technological and scientific developments in the NCFG.

Public Health Relevance

This proposed National Center for Functional Glycomics (NCFG) will provide a unique technological and biological resource for studying the structure and function of human and animal glycomes, through innovative glycan microarray technologies, and novel glycan presentations, as well as explore an important set of driving biological projects that exploit the technology developments. The NCFG will provide a unique national resource in collaboration, training, and dissemination of results in the field that will elevate and propel important advances in glycomics.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
5P41GM103694-02
Application #
8711517
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Program Officer
Sheeley, Douglas
Project Start
2013-08-01
Project End
2018-06-30
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
2
Fiscal Year
2014
Total Cost
$1,088,787
Indirect Cost
$307,512
Name
Emory University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
066469933
City
Atlanta
State
GA
Country
United States
Zip Code
30322
Schneider, Christoph; Wicki, Simone; Graeter, Stefanie et al. (2017) IVIG regulates the survival of human but not mouse neutrophils. Sci Rep 7:1296
Tati, Swetha; Fisk, John C; Abdullah, Julia et al. (2017) Humanization of JAA-F11, a Highly Specific Anti-Thomsen-Friedenreich Pancarcinoma Antibody and InVitro Efficacy Analysis. Neoplasia 19:716-733
Pantophlet, Ralph; Trattnig, Nino; Murrell, Sasha et al. (2017) Bacterially derived synthetic mimetics of mammalian oligomannose prime antibody responses that neutralize HIV infectivity. Nat Commun 8:1601
Sardar, Mohammed Y R; Krishnamurthy, Venkata R; Park, Simon et al. (2017) Synthesis of LewisX-O-Core-1 threonine: A building block for O-linked LewisX glycopeptides. Carbohydr Res 452:47-53
Mahajan, Sonal; Khairnar, Aasawari; Bishnoi, Ritika et al. (2017) Microbial F-type lectin domains with affinity for blood group antigens. Biochem Biophys Res Commun 491:708-713
Taniguchi, Takazumi; Woodward, Ashley M; Magnelli, Paula et al. (2017) N-Glycosylation affects the stability and barrier function of the MUC16 mucin. J Biol Chem 292:11079-11090
Bunker, Jeffrey J; Erickson, Steven A; Flynn, Theodore M et al. (2017) Natural polyreactive IgA antibodies coat the intestinal microbiota. Science 358:
Collins, Bernard C; Gunn, Robin J; McKitrick, Tanya R et al. (2017) Structural Insights into VLR Fine Specificity for Blood Group Carbohydrates. Structure 25:1667-1678.e4
Hamilton, Brian S; Wilson, Joshua D; Shumakovich, Marina A et al. (2017) A library of chemically defined human N-glycans synthesized from microbial oligosaccharide precursors. Sci Rep 7:15907
Mickum, Megan L; Rojsajjakul, Teerapat; Yu, Ying et al. (2016) Schistosoma mansoni ?1,3-fucosyltransferase-F generates the Lewis X antigen. Glycobiology 26:270-85

Showing the most recent 10 out of 59 publications