The mission of the Boston University Mass Spectrometry Research Resource for Biology and Medicine is the development and application of advanced, mass spectrometry-based methods for the characterization of biopolymers that are relevant to human health and disease. Its steady progress has been fostered by close working relationships between basic scientists, clinicians and trainees at the student and postdoctoral levels. During the next grant period, the Resource will carry out Technological Research and Development (TR&D) projects that are designed to address the needs of major collaborators with whom it has identified Driving Biological Projects (DBPs) and will also use the tools and approaches that result from these efforts to solve challenging biological questions raised by additional collaborators and the wider community. The TR&D goal of the BUSM MS Resource is to advance MS methods and instrumentation to meet the short- and long-term needs of medicine. The selection of DBPs aims to identify new areas appropriate for MS in the health sciences, develop new MS-based approaches to meet the requirements of these fields and apply cutting-edge MS to the solution of critical questions in the life sciences. The Resource will focus on the needs of glycobiology for detailed structural elucidations and the profiling of complex mixtures of glycans. In particular, glycan fragmentation pathways will be thoroughly explored using novel electron-based and established dissociation methods and new bioinformatics tools will be developed for glycan analysis. Methods for 2-D analysis of glycans, lipids and proteins on surfaces and in tissues will be developed. Ion mobility mass spectrometry will be investigated for the analysis of glycans, glycoconjugates and complex peptide mixtures and for the characterization of noncovalent complexes. Top-down analysis of variant and post-translationally modified proteins will be pursued to establish relationships among PTMs on individual proteins, to provide straightforward clinical analyses, and to probe protein-glycan and protein-protein interactions. The Resource will train students, postdoctoral fellows and practicing scientists in mass spectrometry, and educate the local, national and international community about modern MS to encourage its wide and appropriate use.
Biological projects will include glycoform profiling of influenza strains, characterization of N- and O-linked glycans and lipopolysaccharides on infectious bacteria and parasites, structural determinations of glycolipids and glycosaminoglycans in human milk that have anti-AlDS activity, protein misfolding disorders, immunology, extracellular matrix biology, cancer and cardiovascular disease;all impact human health.
|Premasiri, W Ranjith; Lee, Jean C; Sauer-Budge, Alexis et al. (2016) The biochemical origins of the surface-enhanced Raman spectra of bacteria: a metabolomics profiling by SERS. Anal Bioanal Chem 408:4631-47|
|Khatri, Kshitij; Klein, Joshua A; White, Mitchell R et al. (2016) Integrated Omics and Computational Glycobiology Reveal Structural Basis for Influenza A Virus Glycan Microheterogeneity and Host Interactions. Mol Cell Proteomics 15:1895-912|
|Chandler, Kevin Brown; Costello, Catherine E (2016) Glycomics and glycoproteomics of membrane proteins and cell-surface receptors: Present trends and future opportunities. Electrophoresis 37:1407-19|
|Hu, Han; Khatri, Kshitij; Zaia, Joseph (2016) Algorithms and design strategies towards automated glycoproteomics analysis. Mass Spectrom Rev :|
|Wang, Yun Hwa Walter; Meyer, Rosana D; Bondzie, Philip A et al. (2016) IGPR-1 Is Required for Endothelial Cell-Cell Adhesion and Barrier Function. J Mol Biol 428:5019-5033|
|Zaia, Joseph; Khatri, Kshitij; Klein, Joshua et al. (2016) Complete Molecular Weight Profiling of Low-Molecular Weight Heparins Using Size Exclusion Chromatography-Ion Suppressor-High-Resolution Mass Spectrometry. Anal Chem 88:10654-10660|
|Dierker, Tabea; Shao, Chun; Haitina, Tatjana et al. (2016) Nematodes join the family of chondroitin sulfate-synthesizing organisms: Identification of an active chondroitin sulfotransferase in Caenorhabditis elegans. Sci Rep 6:34662|
|Pu, Yi; Ridgeway, Mark E; Glaskin, Rebecca S et al. (2016) Separation and Identification of Isomeric Glycans by Selected Accumulation-Trapped Ion Mobility Spectrometry-Electron Activated Dissociation Tandem Mass Spectrometry. Anal Chem 88:3440-3|
|He, Huan; Liu, Dan; Lin, Hui et al. (2016) Phosphatidylethanolamine binding protein 4 (PEBP4) is a secreted protein and has multiple functions. Biochim Biophys Acta 1863:1682-9|
|Aphasizheva, Inna; Maslov, Dmitri A; Qian, Yu et al. (2016) Ribosome-associated pentatricopeptide repeat proteins function as translational activators in mitochondria of trypanosomes. Mol Microbiol 99:1043-58|
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