In the last decade the NIH has invested billions of dollars to place cutting-edge MS instrumentation in laboratories across the US. Several other Federal entities, along with private and academic institutions, have likewise contributed, such that a research institute without at least one state-of-the-art MS system is now the exception to the rule. For all this investment, biomedical researchers lacking MS expertise can now submit samples, mostly to core facilities but also to expert collaborators, for protein analysis. This analysis most often results in a list of proteins present in a sample. More seasoned facilities wil have in-house expertise in PTM analysis and may, for example, offer phosphorylation site analysis on targeted proteins or complex mixtures. Considering where the field of proteomics was just fifteen years ago-low throughput peptide mass mapping from gel bands-broad access to today's protein analysis is remarkable. Doubtless this success stems from the sizeable investment of NIH and other funding agencies in fundamental technology development. That said, global protein quantification methodologies, either relative or absolute, are not routine even for most expert laboratories. The result is highly rationed access to arguably the most valuable and telling type of proteomic data. Understanding the networks that regulate complex organisms and their diseases will require wide and pervasive access to these critical comprehensive technologies. The NCQBCS will catalyze and expedite this transformation in quantitative biology.

Public Health Relevance

The National Center for Quantitative Biology of Complex Systems seeks to develop next-generation protein measurement technologies and to make these methods accessible to the broad community. These essential technologies will be developed in the context of a cadre of Driving Biomedical Projects (DBPs). The DBP diversity ensures that the NCQBCS will fulfill its most critical mission: to empower all biomedical researchers, with their sundry models and samples by advancing and making accessible protein quantification technologies.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Biotechnology Resource Grants (P41)
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Special Emphasis Panel (ZRG1-BCMB-P (40)P)
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Sheeley, Douglas
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University of Wisconsin Madison
Schools of Medicine
United States
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Chung, Jacky; Wittig, Johannes G; Ghamari, Alireza et al. (2017) Erythropoietin signaling regulates heme biosynthesis. Elife 6:
Peuchen, Elizabeth H; Cox, Olivia F; Sun, Liangliang et al. (2017) Phosphorylation Dynamics Dominate the Regulated Proteome during Early Xenopus Development. Sci Rep 7:15647
Guo, Xiao; Niemi, Natalie M; Hutchins, Paul D et al. (2017) Ptc7p Dephosphorylates Select Mitochondrial Proteins to Enhance Metabolic Function. Cell Rep 18:307-313
Yu, Qing; Shi, Xudong; Feng, Yu et al. (2017) Improving data quality and preserving HCD-generated reporter ions with EThcD for isobaric tag-based quantitative proteomics and proteome-wide PTM studies. Anal Chim Acta 968:40-49
Liu, Fabao; Ma, Fengfei; Wang, Yuyuan et al. (2017) PKM2 methylation by CARM1 activates aerobic glycolysis to promote tumorigenesis. Nat Cell Biol 19:1358-1370
Veling, Mike T; Reidenbach, Andrew G; Freiberger, Elyse C et al. (2017) Multi-omic Mitoprotease Profiling Defines a Role for Oct1p in Coenzyme Q Production. Mol Cell 68:970-977.e11
Lapointe, Christopher P; Stefely, Jonathan A; Jochem, Adam et al. (2017) Multi-omics Reveal Specific Targets of the RNA-Binding Protein Puf3p and Its Orchestration of Mitochondrial Biogenesis. Cell Syst :
Hao, Ling; Johnson, Jillian; Lietz, Christopher B et al. (2017) Mass Defect-Based N,N-Dimethyl Leucine Labels for Quantitative Proteomics and Amine Metabolomics of Pancreatic Cancer Cells. Anal Chem 89:1138-1146
Hewitt, Kyle J; Katsumura, Koichi R; Matson, Daniel R et al. (2017) GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia. Dev Cell 42:213-225.e4
Diaz, Juan E; Morgan, Charles W; Minogue, Catherine E et al. (2017) A Split-Abl Kinase for Direct Activation in Cells. Cell Chem Biol 24:1250-1258.e4

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