The project will develop a world-leading suite of experimental facilities for the life-science research community. With the focus on macromolecular crystallography (MX) and x-ray scattering, there will also be user programs in correlated MX and optical spectroscopy, and one in fluorescence imaging of metals in biological materials. The extraordinary brightness of NSLS-II will provide unprecedentedly small, bright, and well-collimated x-ray beams. To exploit these qualities, extra care will be exerted to provide the best quality crystal-diffraction and x-ray scattering data from the NSLS-II beams, and high levels of automation. Multiple experimental modes will be installed for x-ray scattering to investigate solutions, membranes, and tissues. We expect high success of the project, owed in part to the remarkable community of life-science researchers in the 400 mile-long corridor from Boston to Washington, all within driving distance of BNL, and to the solid record of productivity in structural biology having been provided this community during the lifetime of the NSLS.

Public Health Relevance

Synchrotron x-ray sources provide the best possible structural information for biologists active in medical, and all other life-science, research. NSLS II will be the brightest source available when it comes into operation in 2015-16. This project wil provide the best possible experimental facilities to enable life scientists to employ the facility or their research.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-IMST-K (40))
Program Officer
Swain, Amy L
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Brookhaven National Laboratory
United States
Zip Code
Hsu, Hao-Chi; Singh, Pradeep K; Fan, Hao et al. (2017) Structural Basis for the Species-Selective Binding of N,C-Capped Dipeptides to the Mycobacterium tuberculosis Proteasome. Biochemistry 56:324-333
Popelka, Hana; Damasio, Alejandro; Hinshaw, Jenny E et al. (2017) Structure and function of yeast Atg20, a sorting nexin that facilitates autophagy induction. Proc Natl Acad Sci U S A 114:E10112-E10121
Guan, Rongjin; Aiyer, Sriram; Cote, Marie L et al. (2017) X-ray crystal structure of the N-terminal region of Moloney murine leukemia virus integrase and its implications for viral DNA recognition. Proteins 85:647-656
Han, Qian; Wong, Dawn M; Robinson, Howard et al. (2017) Crystal structure of acetylcholinesterase catalytic subunits of the malaria vector Anopheles gambiae. Insect Sci :
Singh, Abhay Kumar; Pantouris, Georgios; Borosch, Sebastian et al. (2017) Structural basis for decreased induction of class IB PI3-kinases expression by MIF inhibitors. J Cell Mol Med 21:142-153
Orlova, Natalia; Gerding, Matthew; Ivashkiv, Olha et al. (2017) The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators. Nucleic Acids Res 45:3724-3737
Hangasky, John A; Taabazuing, Cornelius Y; Martin, Cristina B et al. (2017) The facial triad in the ?-ketoglutarate dependent oxygenase FIH: A role for sterics in linking substrate binding to O2 activation. J Inorg Biochem 166:26-33
Cheung, Kalung; Lu, Geming; Sharma, Rajal et al. (2017) BET N-terminal bromodomain inhibition selectively blocks Th17 cell differentiation and ameliorates colitis in mice. Proc Natl Acad Sci U S A 114:2952-2957
Li, Xiaofeng; Tao, Yeqing; Murphy, James W et al. (2017) The repeat region of cortactin is intrinsically disordered in solution. Sci Rep 7:16696
Fuller, Franklin D; Gul, Sheraz; Chatterjee, Ruchira et al. (2017) Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. Nat Methods 14:443-449

Showing the most recent 10 out of 204 publications