To assure that the M.I.T. Mass Spectrometry Facility remains in the forefront of the field the area of research will be expanded to large polar molecules (up to l0,000 daltons and possibly beyond). Methods for the ionization of such molecules will be improved. Major emphasis will be on """"""""soft"""""""" ionization techniques such as field desorption, fast atom bombardment and direct chemical ionization applied to large, polar molecules of biological interest. Laser-induced fragmentation will be investigated as a means for deriving more structural information. Proteins, oligopeptides, nucleotides, membrane structure, sphingo lipids, drug metabolites are major areas of interest. This methodology will be made available to the biomedical community through collaboration or as a service.
Biemann, Klaus (2015) Structure Determination of Natural Products by Mass Spectrometry. Annu Rev Anal Chem (Palo Alto Calif) 8:1-19 |
Biemann, Klaus (2014) Laying the groundwork for proteomics: mass spectrometry from 1958 to 1988. J Proteomics 107:62-70 |
Brennan, Mary R; Costello, Catherine E; Maleknia, Simin D et al. (2008) Stylopeptide 2, a proline-rich cyclodecapeptide from the sponge Stylotella sp. J Nat Prod 71:453-6 |
Perreault, H; Costello, C E (1999) Stereochemical effects on the mass spectrometric behavior of native and derivatized trisaccharide isomers: comparisons with results from molecular modeling. J Mass Spectrom 34:184-97 |
Costello, C E (1997) Time, life ... and mass spectrometry. New techniques to address biological questions. Biophys Chem 68:173-88 |
Costello, C E; Glushka, J; van Halbeek, H et al. (1993) Structural characterization of novel inositol phosphosphingolipids of Tritrichomonas foetus and Trichomonas vaginalis. Glycobiology 3:261-9 |