This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This year, our laboratory hosted a workshop on tomography for 14 visitors from around the country and the world. American participants included people from the Burnham Institute, Harvard University, the Wadsworth Center, Arizona State University, University of California at San Diego, the National Institutes of Health, the University of California at Davis and Brandeis University. Foreign participants included visitors from the University of Manchester, England, two from different Max Planck Institutes, one from the Weizmann Institute in Israel, one from the University of Helsinki, and one from Utrecht. These participants heard lectures by David Mastronarde on the theory and practice of tomography, and they had extensive hands on instruction in the use of IMOD, taking advantage of our graphical user interface, eTomo. Instruction in software use was provided by essentially all members of the laboratory, and thanks to the fact that many participants brought their own high quality laptops, sufficient computational facilities were available for everyone to carry out each of the processes involved in converting a series of tilted views into a 3-D reconstruction. Time was also available for people to use the microscopes to experience serial EM and practice data collection. The responses of our visitors to this workshop were extremely enthusiastic, as indicated both by questionnaires filled out at the time and letters of thanks returned by the participants after they went back to their own laboratories. We are encouraged that this format of approximately 14 visitors is something we can handle well and through it make a real contribution to the dissemination of tomography technology to a variety of other labs.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000592-41
Application #
8362533
Study Section
Special Emphasis Panel (ZRG1-CB-J (40))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
41
Fiscal Year
2011
Total Cost
$21,276
Indirect Cost
Name
University of Colorado at Boulder
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
007431505
City
Boulder
State
CO
Country
United States
Zip Code
80309
Giddings Jr, Thomas H; Morphew, Mary K; McIntosh, J Richard (2017) Preparing Fission Yeast for Electron Microscopy. Cold Spring Harb Protoc 2017:pdb.prot091314
Zhao, Xiaowei; Schwartz, Cindi L; Pierson, Jason et al. (2017) Three-Dimensional Structure of the Ultraoligotrophic Marine Bacterium ""Candidatus Pelagibacter ubique"". Appl Environ Microbiol 83:
Brown, Joanna R; Schwartz, Cindi L; Heumann, John M et al. (2016) A detailed look at the cytoskeletal architecture of the Giardia lamblia ventral disc. J Struct Biol 194:38-48
Saheki, Yasunori; Bian, Xin; Schauder, Curtis M et al. (2016) Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nat Cell Biol 18:504-15
Höög, Johanna L; Lacomble, Sylvain; Bouchet-Marquis, Cedric et al. (2016) 3D Architecture of the Trypanosoma brucei Flagella Connector, a Mobile Transmembrane Junction. PLoS Negl Trop Dis 10:e0004312
Park, J Genevieve; Palmer, Amy E (2015) Properties and use of genetically encoded FRET sensors for cytosolic and organellar Ca2+ measurements. Cold Spring Harb Protoc 2015:pdb.top066043
McCoy, Kelsey M; Tubman, Emily S; Claas, Allison et al. (2015) Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles. Mol Biol Cell 26:3999-4014
Höög, Johanna L; Lötvall, Jan (2015) Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. J Extracell Vesicles 4:28680
Marc, Robert E; Anderson, James R; Jones, Bryan W et al. (2014) The AII amacrine cell connectome: a dense network hub. Front Neural Circuits 8:104
Weber, Britta; Tranfield, Erin M; Höög, Johanna L et al. (2014) Automated stitching of microtubule centerlines across serial electron tomograms. PLoS One 9:e113222

Showing the most recent 10 out of 84 publications