This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Instrument maintenance performed on the 1. Perseptive STR reflectron MALDI time-of-flight mass spectrometer 2. Thermo-Finnigan LTQ electrospray ionization ion trap mass spectrometer fitted with on-line HPLC, and ETD fragmentation facility. 3. Thermo-Finnigan LTQ ion trap mass spectrometer fitted with a vacuum MALDI ion source. *4. Finnigan LCQ-DECA-XP electrospray ionization ion trap mass spectrometer fitted with an in-house constructed MALDI ion source incorporating our CD sample inlet system. 5. Finnigan LCQ-DECA electrospray ionization ion trap mass spectrometer fitted with an on-line microflow HPLC separation unit. 6. Waters Synapt Ion mobility Quadrupole Time-of-Flight Mass Spectrometer *7. Prototype novel high-capacity ion trap-quadrupole tandem mass spectrometer fitted to a prototype Sciex QQTOF mass spectrometer fitted with an in-house constructed MALDI ion source incorporating our automated CD sample inlet system. 8. Perkin Elmer ProTOF orthogonal Injection Mass Spectrometer 9. Finnigan ETD-LTQ-Orbitrap Mass Spectrometer 10. Pharmacia-Amersham Smart HPLC system 11. LC Packings HPLC system 12. Micro-Tech Scientific XtremeSimple HPLC system. 13 Two Agilent HPLC systems Service in the mass spectrometers included maintenance of vacuum pumps, cleaning of the ion source, replacement of ion detectors, repair of electronics;the HPLC systems required maintenance of piston and syringe pumps and replacement of seals, check valves, and trouble shooting.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000862-38
Application #
8361487
Study Section
Special Emphasis Panel (ZRG1-BCMB-Q (40))
Project Start
2011-03-01
Project End
2012-03-31
Budget Start
2011-03-01
Budget End
2012-03-31
Support Year
38
Fiscal Year
2011
Total Cost
$5,214
Indirect Cost
Name
Rockefeller University
Department
Miscellaneous
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
Manning, Lois R; Popowicz, Anthony M; Padovan, Julio C et al. (2017) Gel filtration of dilute human embryonic hemoglobins reveals basis for their increased oxygen binding. Anal Biochem 519:38-41
Chait, Brian T; Cadene, Martine; Olinares, Paul Dominic et al. (2016) Revealing Higher Order Protein Structure Using Mass Spectrometry. J Am Soc Mass Spectrom 27:952-65
Boice, Michael; Salloum, Darin; Mourcin, Frederic et al. (2016) Loss of the HVEM Tumor Suppressor in Lymphoma and Restoration by Modified CAR-T Cells. Cell 167:405-418.e13
Krutchinsky, Andrew N; Padovan, Júlio C; Cohen, Herbert et al. (2015) Maximizing ion transmission from atmospheric pressure into the vacuum of mass spectrometers with a novel electrospray interface. J Am Soc Mass Spectrom 26:649-58
Mast, Fred D; Rachubinski, Richard A; Aitchison, John D (2015) Signaling dynamics and peroxisomes. Curr Opin Cell Biol 35:131-6
Krutchinsky, Andrew N; Padovan, Júlio C; Cohen, Herbert et al. (2015) Optimizing electrospray interfaces using slowly diverging conical duct (ConDuct) electrodes. J Am Soc Mass Spectrom 26:659-67
Oricchio, Elisa; Papapetrou, Eirini P; Lafaille, Fabien et al. (2014) A cell engineering strategy to enhance the safety of stem cell therapies. Cell Rep 8:1677-1685
Zhong, Yu; Morris, Deanna H; Jin, Lin et al. (2014) Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. J Biol Chem 289:26021-37
Xue, John Z; Woo, Eileen M; Postow, Lisa et al. (2013) Chromatin-bound Xenopus Dppa2 shapes the nucleus by locally inhibiting microtubule assembly. Dev Cell 27:47-59
Indiani, Chiara; O'Donnell, Mike (2013) A proposal: Source of single strand DNA that elicits the SOS response. Front Biosci (Landmark Ed) 18:312-23

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