This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Alzheimer?s disease (AD) is a neurodegenerative disease with progressive loss of brain cells as well as learning and memory performance in eldly population. Studies have found that one of the pathological hallmarks for this disease is accumulation of the senile plaques, a pathological stains consisting of junk proteins, in the brain tissues. However, this type of plaques can now only be detected uisng autopsied tissues. Interestingly, we recently found that Alzheimer's-related senile plaques are also present in the retinas of transgenic mice with AD. The objective of this pilot study is to develop an imaging technique using the modified optical coherance tomography (mOCT) to detect senile plaques in the retinas in live mice with AD. Accordingly, a transgenic AD mouse will be given a small amout of non-toxic fluorescent dye or a dye-labelled antibody to stain the plaques prior to imaging. Fluorescent retinal fundus images will be then obtained using mOCT from the mouse under anesthesia. Once the imaging procedure is completed, both brains and eyes of the mice will be colleted for pathological analysis following euthansia. At last, the information collected from the retinal images will be correlated with these pathological observations. Anticipated results from proposed studies would help us to develop a new imaging technique to detect Alzheimer's plaques through eyes, which will provide a powerful tool for early diagnosis of AD that can be readily translated to the clinical setting

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001192-32
Application #
8362696
Study Section
Special Emphasis Panel (ZRG1-SBIB-L (40))
Project Start
2011-04-01
Project End
2012-03-31
Budget Start
2011-04-01
Budget End
2012-03-31
Support Year
32
Fiscal Year
2011
Total Cost
$3,112
Indirect Cost
Name
University of California Irvine
Department
Physiology
Type
Schools of Medicine
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Takesh, Thair; Sargsyan, Anik; Lee, Matthew et al. (2017) Evaluating the Whitening and Microstructural Effects of a Novel Whitening Strip on Porcelain and Composite Dental Materials. Dentistry (Sunnyvale) 7:
Jonscher, Karen R; Stewart, Michael S; Alfonso-Garcia, Alba et al. (2017) Early PQQ supplementation has persistent long-term protective effects on developmental programming of hepatic lipotoxicity and inflammation in obese mice. FASEB J 31:1434-1448
Takesh, Thair; Sargsyan, Anik; Anbarani, Afarin et al. (2017) Effects of a Novel Whitening Formulation on Dental Enamel. Dentistry (Sunnyvale) 7:
Alfonso-García, Alba; Paugh, Jerry; Farid, Marjan et al. (2017) A machine learning framework to analyze hyperspectral stimulated Raman scattering microscopy images of expressed human meibum. J Raman Spectrosc 48:803-812
Alfonso-García, Alba; Pfisterer, Simon G; Riezman, Howard et al. (2016) D38-cholesterol as a Raman active probe for imaging intracellular cholesterol storage. J Biomed Opt 21:61003
Malacrida, Leonel; Astrada, Soledad; Briva, Arturo et al. (2016) Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. Biochim Biophys Acta 1858:2625-2635
Choi, Bernard; Tan, Wenbin; Jia, Wangcun et al. (2016) The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities. IEEE J Sel Top Quantum Electron 2016:
Wang, Mingqiu; Ravindranath, Shreyas R; Rahim, Maha K et al. (2016) Evolution of Multivalent Nanoparticle Adhesion via Specific Molecular Interactions. Langmuir 32:13124-13136
Alfonso-García, Alba; Smith, Tim D; Datta, Rupsa et al. (2016) Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy. J Biomed Opt 21:46005
Prince, Richard C; Frontiera, Renee R; Potma, Eric O (2016) Stimulated Raman Scattering: From Bulk to Nano. Chem Rev :

Showing the most recent 10 out of 659 publications