Small angle x-ray scattering studies will be performed on an endonuclease protein (FEN-1). This protein is important in DNA replication and damage repair. It recognizes a specific type of branch defect in the DNA which it specifically repairs. DNA binding and repair seem to be separate properties of this protein. We also have mutants which can still bind DNA but have lost their repair ability. Also, repair requires magnesium to be present. We intend to measure 4 samples, the wild type (+/- Mg) and the repair inactivated mutant (+/- Mg). A high-flux synchrotron beam and an adequate low-background SAXS facility are necessary to get enough data statistics from these dilute solution samples (approx. 1.5 mg/ml).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-19
Application #
2788413
Study Section
Project Start
Project End
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
19
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
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