Initial x-ray absorption studies performed under Rapid Turnaround XAS proposal 1E23 showed that XAS is sensitive to the different Cd environments on the cell wall and in a metal-binding polypeptide from metal-tolerant Datura innoxia tissue culture. These studies suggest that it may be possible to use XAS to follow the in situ transformation of heavy metals by metal-accumulating plants. Based on these results, we propose to extend this work to characterize the Ni and Cr sites on D. innoxia cell walls and in metal complexes isolated from D. innoxia plant cells. We also propose to use XAS to follow the in situ transformation of Cd by D. innoxia tissue culture. These studies will provide insight into the transformation of metal ions by metal-tolerant plants on a cellular level, and contribute to an understanding of the biotic transformation pathways that contribute to the fate of metal ions in the environment.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-22
Application #
6437685
Study Section
Project Start
2001-03-01
Project End
2002-02-28
Budget Start
Budget End
Support Year
22
Fiscal Year
2001
Total Cost
$143,176
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
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