This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The SSRL SMB Center staff provides each user group hands-on training on the effective use of the SMB SAXS/D instrument. All users first receive a brief safety orientation specific to BL4-2 and an overview of the instrument, including updates on recent equipment changes. The staff discuss user experimental requirements, configure the instrument as necessary and train users. Users receive a tutorial on Blu-Ice software used for all types of experiment on BL4-2 with particular emphasis on a specific data collection ?tab? required for their data collection, for instance the solSAXS tab for solution scattering studies. They also receive instruction on sample-handling equipment and a tutorial on SAStool, the data processing program for non-crystallined diffraction studies developed by the SMB staff. Users are instructed on initial steps of data analysis, such as inspecting data for possible radiation-induced aggregation and assessing data quality. The staff is also available to help users set up data collection strategies. The data processing program Primus, developed by an EMBL-Hamburg group, is used for data display and computing basic structural parameters such as radii of gyration (Rg). The BTP staff walks users through all the above by measuring first set of x-ray scattering data together. The staff also gives a short tutorial on Fit2D, developed at the European Synchrotron Radiation Facility, upon request. These hands-on training sessions are given to new as well as experienced user groups.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-32
Application #
8362072
Study Section
Special Emphasis Panel (ZRG1-BCMB-P (40))
Project Start
2011-03-01
Project End
2012-02-29
Budget Start
2011-03-01
Budget End
2012-02-29
Support Year
32
Fiscal Year
2011
Total Cost
$27,423
Indirect Cost
Name
Stanford University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
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