This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Multi-edge x-ray absorption spectroscopic investigations of modified Fe-S mineral surfaces and nanoparticles are proposed in order to map the Fe-S phase diagrams as a function of length scale (micrometer vs. nanometer), synthesis (surface/beam, protein-cage controlled synthesis), and preparation conditions (exposure time, temperature, pH, presence of other ions). We hypothesize that using energy- (surface/beam) and structure- (protein cage or viral capsid) controlled synthesis of Fe-S systems, areas of Fe-S phase diagrams can be accessed that would not be possible by conventional synthetic methods. Recently obtained preliminary data already indicate the existence of a previously unobserved reduced state of pyrite, which was prepared by exposing pristine [100] pyrite surface to hydrogen atom/hydrogen gas plasma. We will take advantage of the complementarity of XAS data at the Fe K-edge, Fe L-edge, and S K-edge. Recent beamline development at BL4-3 now allows for collection of sulfur EXAFS, which in combination with iron EXAFS can provide more reliable fits of geometric structure for hard to characterize intermediates or transition states. XANES analysis of preedge and rising-edge features of Fe L-edge and S K-edge spectra provides electronic structural information about effective nuclear charges of metal/ligand centers, orbital compositions, ligand-field splitting of metal centers, and non-innocent behavior of ligands. Structural insights from the proposed experiments have an impact on the role of Fe-S systems as pre-biotic metabolic catalysts (NASA Astrobiology Institute supported research program at MSU), as well as, potential in proposing novel industrial catalysts from abundant Fe-S containing materials for activation of small molecules such as dinitrogen or dihydrogen.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BCMB-P (40))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Stanford University
Schools of Arts and Sciences
United States
Zip Code
Batyuk, Alexander; Galli, Lorenzo; Ishchenko, Andrii et al. (2016) Native phasing of x-ray free-electron laser data for a G protein-coupled receptor. Sci Adv 2:e1600292
Lins, Brittney R; Pushie, Jake M; Jones, Michael et al. (2016) Mapping Alterations to the Endogenous Elemental Distribution within the Lateral Ventricles and Choroid Plexus in Brain Disorders Using X-Ray Fluorescence Imaging. PLoS One 11:e0158152
Sierra, Raymond G; Gati, Cornelius; Laksmono, Hartawan et al. (2016) Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II. Nat Methods 13:59-62
Cowley, Ryan E; Cirera, Jordi; Qayyum, Munzarin F et al. (2016) Structure of the Reduced Copper Active Site in Preprocessed Galactose Oxidase: Ligand Tuning for One-Electron O2 Activation in Cofactor Biogenesis. J Am Chem Soc 138:13219-13229
Edlund, Petra; Takala, Heikki; Claesson, Elin et al. (2016) The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography. Sci Rep 6:35279
McLuskey, Karen; Grewal, Jaspreet S; Das, Debanu et al. (2016) Crystal Structure and Activity Studies of the C11 Cysteine Peptidase from Parabacteroides merdae in the Human Gut Microbiome. J Biol Chem 291:9482-91
Colletier, Jacques-Philippe; Sawaya, Michael R; Gingery, Mari et al. (2016) De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure. Nature 539:43-47
Agrawal, Anant A; Salsi, Enea; Chatrikhi, Rakesh et al. (2016) An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal. Nat Commun 7:10950
Kaever, Thomas; Matho, Michael H; Meng, Xiangzhi et al. (2016) Linear Epitopes in Vaccinia Virus A27 Are Targets of Protective Antibodies Induced by Vaccination against Smallpox. J Virol 90:4334-45
Nogly, Przemyslaw; Panneels, Valerie; Nelson, Garrett et al. (2016) Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. Nat Commun 7:12314

Showing the most recent 10 out of 563 publications