: The NIH Roadmap Initiative has as one of its key underlying objectives the implementation of high through put, multiplexed instruments for application in a wide variety of basic and clinical research. The flow cytometry platform is ideally suited to this purpose, and several recent technologies have significantly increased throughput and simultaneous, multiparametric analysis. We propose to provide several critical technology advances by extending the capabilities and applications of flow-based analysis and sorting to produce an increasingly multiparameter, high-throughput analysis and screening tool. This will be accomplished through an integrated plan of three Research and Development Projects, extensive internal and external Collaborations, a wide variety of Service projects, expansion of our Training efforts, and increased Dissemination activities. The R&D Projects aim to extend flow cytometry into three areas of direct relevance to the Roadmap goals: 1) application of acoustic focusing and sensing technology to provide both novel analysis parameters and in-line sample preparation;2) increasing both the throughput and the particle size range of flow sorting technologies;and 3) developing an integrated instrument to collect complete emission spectra separated according to fluorescence lifetime. Each of these projects has a defined set of collaborations that apply the new technologies to a wide range of biomedical research, including protein engineering, protein-protein interactions, drug and ligand screening, multiplexed cellular analysis, and automated preparation and analysis of complex clinical and environmental samples. In addition, we will continue and expand our Collaborative projects using instruments developed in the previous funding period. We will expand our Service efforts by providing access to a wide variety of commercial and unique developmental instruments. We will continue our traditionally outstanding Training program of hands-on courses and student/PostDoc mentorship, and develop new courses focused on application of our recent technology advances. Our strong Dissemination program will also be extended to include an improved website and the sponsorship of three focused meetings on various aspects of advanced flow cytometry. In conclusion, we propose to continue to drive the design, development, and application of flow cytometry technology as a multiplexed, high-throughput platform for advanced biomedical and clinical research.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001315-29
Application #
7802941
Study Section
Special Emphasis Panel (ZRG1-CB-K (40))
Program Officer
Sheeley, Douglas
Project Start
1997-08-26
Project End
2012-03-31
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
29
Fiscal Year
2010
Total Cost
$1,705,221
Indirect Cost
Name
Los Alamos National Lab
Department
Type
DUNS #
175252894
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
Johnson, Leah M; Gao, Lu; Shields IV, C Wyatt et al. (2013) Elastomeric microparticles for acoustic mediated bioseparations. J Nanobiotechnology 11:22
Ai, Ye; Sanders, Claire K; Marrone, Babetta L (2013) Separation of Escherichia coli bacteria from peripheral blood mononuclear cells using standing surface acoustic waves. Anal Chem 85:9126-34
Micheva-Viteva, Sofiya N; Shou, Yulin; Nowak-Lovato, Kristy L et al. (2013) c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response. BMC Microbiol 13:249
Sanders, Claire K; Mourant, Judith R (2013) Advantages of full spectrum flow cytometry. J Biomed Opt 18:037004
Cushing, Kevin W; Piyasena, Menake E; Carroll, Nick J et al. (2013) Elastomeric negative acoustic contrast particles for affinity capture assays. Anal Chem 85:2208-15
Piyasena, Menake E; Austin Suthanthiraraj, Pearlson P; Applegate Jr, Robert W et al. (2012) Multinode acoustic focusing for parallel flow cytometry. Anal Chem 84:1831-9
Austin Suthanthiraraj, Pearlson P; Piyasena, Menake E; Woods, Travis A et al. (2012) One-dimensional acoustic standing waves in rectangular channels for flow cytometry. Methods 57:259-71
Vuyisich, Momchilo; Sanders, Claire K; Graves, Steven W (2012) Binding and cell intoxication studies of anthrax lethal toxin. Mol Biol Rep 39:5897-903
Chaudhary, Anu; Ganguly, Kumkum; Cabantous, Stephanie et al. (2012) The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochem Biophys Res Commun 417:299-304
Marina, Oana C; Sanders, Claire K; Mourant, Judith R (2012) Effects of acetic acid on light scattering from cells. J Biomed Opt 17:085002-1

Showing the most recent 10 out of 239 publications