This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent FTIR investigations of the OH stretch mode of water in guanidine solutions revealed a large frequency shift to lower frequency of the O-H stretch mode after guanidine addition, arising questions about the structure of the solvation shell of water molecules around the guanidine ions. Guanidine is often used as a protein denaturant. It may act as a denaturant because it binds to proteins directly since it has 3 NH2 groups that mimic water molecules in their H-bonding ability. The goal of this project is to generate a microscopic description of the interaction between guanidine and water and deduce why guanidine is so effective as a denaturant in biology. It has been postulated that guanidine changes the structure of the water network. By using dual frequency 2D-IR, we are able to visualize the interaction between guanidine and the surrounding water as well as the water-water interactions. By using 2D-IR, we should be able to tell between the following: Are the water molecules surrounding guanidine isolated from the bulk water? Do the frequency fluctuations of the OH stretch modes of the solvation shell manifest coupling to the ion? Or are the water molecules surrounding guanidine similar in their physical properties to the bulk water?

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001348-30
Application #
8362573
Study Section
Special Emphasis Panel (ZRG1-BCMB-N (40))
Project Start
2011-06-01
Project End
2012-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
30
Fiscal Year
2011
Total Cost
$19,509
Indirect Cost
Name
University of Pennsylvania
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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