This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Bioactive lipids, such as prostaglandin E2 (PGE2), are signaling molecules generated by cells of the immune system to regulate inflammation in humans. Bioactive lipids are also biosynthesized by fungi, such as the pathogen Candida albicans and the saprophyte Saccharomyces cerevisiae (brewers yeast). The latter is employed to manufacture a wide range of foods, pharmaceuticals and vaccines.
We aim to identify the categories of products consumed by humans that contain bioactive lipids produced by S. cerevisiae and define the biosynthetic pathway(s) used by the fungus to produce bioactive lipids. Understanding how fungi generate bioactive lipids will inform of strategies for limiting bioactive lipid biosynthesis during product manufacture. The proposed research is initially focused upon PGE2 because it is one of the better-understood bioactive lipids and is produced by S. cerevisiae. The outcome measure of this study will be determining whether bioactive lipids are absent or present in select products manufactured using S. cerevisiae. The UCSF Mass Spectrometry Facility can assist in obtaining our research goals by analyzing extracted lipids from select products for the presence of bioactive lipids such as PGE2, using liquid chromatography-mass spectrometry (LC-MS).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001614-29
Application #
8363806
Study Section
Special Emphasis Panel (ZRG1-BCMB-M (40))
Project Start
2011-06-01
Project End
2012-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
29
Fiscal Year
2011
Total Cost
$4,357
Indirect Cost
Name
University of California San Francisco
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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