This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Previous experiments at D1 have demonstrated the feasibility of collecting Laue data from protein crystals down to about 30 microns in size, at both cryo and room temperature, using a wide-bandpass beam. We request time at G1 to: (1) compare data quality with wide (25%) and narrow (1.5%) bandpasses, (2) see whether useful data can be obtained from crystals of <10 microns size using the intense wiggler beam at G1, (3) attempt to obtain anomalous data from proteins containing zinc, for SAD phasing.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001646-29
Application #
8363576
Study Section
Special Emphasis Panel (ZRG1-BCMB-E (40))
Project Start
2011-07-01
Project End
2012-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
29
Fiscal Year
2011
Total Cost
$33,020
Indirect Cost
Name
Cornell University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Bauman, Joseph D; Harrison, Jerry Joe E K; Arnold, Eddy (2016) Rapid experimental SAD phasing and hot-spot identification with halogenated fragments. IUCrJ 3:51-60
Xu, Caishuang; Kozlov, Guennadi; Wong, Kathy et al. (2016) Crystal Structure of the Salmonella Typhimurium Effector GtgE. PLoS One 11:e0166643
Cogliati, Massimo; Zani, Alberto; Rickerts, Volker et al. (2016) Multilocus sequence typing analysis reveals that Cryptococcus neoformans var. neoformans is a recombinant population. Fungal Genet Biol 87:22-9
Oot, Rebecca A; Kane, Patricia M; Berry, Edward A et al. (2016) Crystal structure of yeast V1-ATPase in the autoinhibited state. EMBO J 35:1694-706
Lucido, Michael J; Orlando, Benjamin J; Vecchio, Alex J et al. (2016) Crystal Structure of Aspirin-Acetylated Human Cyclooxygenase-2: Insight into the Formation of Products with Reversed Stereochemistry. Biochemistry 55:1226-38
Gupta, Kushol; Martin, Renee; Sharp, Robert et al. (2015) Oligomeric Properties of Survival Motor Neuron·Gemin2 Complexes. J Biol Chem 290:20185-99
Moravcevic, Katarina; Alvarado, Diego; Schmitz, Karl R et al. (2015) Comparison of Saccharomyces cerevisiae F-BAR domain structures reveals a conserved inositol phosphate binding site. Structure 23:352-63
Orlando, Benjamin J; Lucido, Michael J; Malkowski, Michael G (2015) The structure of ibuprofen bound to cyclooxygenase-2. J Struct Biol 189:62-6
Wong, Kathy; Kozlov, Guennadi; Zhang, Yinglu et al. (2015) Structure of the Legionella Effector, lpg1496, Suggests a Role in Nucleotide Metabolism. J Biol Chem 290:24727-37
Muñoz-Escobar, Juliana; Matta-Camacho, Edna; Kozlov, Guennadi et al. (2015) The MLLE domain of the ubiquitin ligase UBR5 binds to its catalytic domain to regulate substrate binding. J Biol Chem 290:22841-50

Showing the most recent 10 out of 368 publications