The Brookhaven Synchrotron X-Ray Microprobe Research Resource uses high-energy x-rays produced by the National Synchrotron Light Source (NSLS) for sensitive trace-element analysis and imaging in various types of biological structures. The NSLS is a uniquely bright source of x-rays and thereby serves as the foundation of the Resources which is presently unsurpassed at any other location.
The aim of the Resource is to combine scientists from the life and physical sciences in a multidisciplinary effort to push the potentialities of synchrotron radiation for elemental x-ray fluorescence measurements to (1) the furthest possible limits in terms of sensitivity, spatial resolutions, and chemical form of the elements examined and (2) to carry on an extensive biological research program to exploit the results of the technical development program. Other Brookhaven nuclear facilities are used to complement the features of the photon beams at the NSLS so as to provide other nuclear analytical techniques when necessary. The complete facility fills the gap between high resolution electron microscopy and bulk analysis techniques. The combination of the synchrotron with computed tomography techniques extends the possibilities of tomography into many new research regions. This Resource uses radiation from a bending magnet to provide filtered white light or monochromatic radiation. The addition of focussing mirrors to the system will give higher fluxes at the target and increase the sensitivity of the determinations. Two different types of mirrors are planned: a simple 1:1 cylindrical mirror and an 8:1 ellipsoidal mirror which focusses in two dimensions. The goal is to obtain detection sensitivities less than 50-100 ppb (by weight) in a 10- to 20-magnetic moment. The Resource presently can be used with a spot size of 25 magnetic moment, and a detection sensitivity around 200 ppb for elements around iron. The initial biological research effort is aimed at studying several aspects of trace element biology and toxicology: (1) trace element localization in the hepatic lobule, (2) trace element interactions, (3) trace element analysis of rodent cerebellium, (4) sterological analysis of microprobe data, and (5) in-vivo analysis of lead in the tibia. A number of collaborative projects in related analytical and biological fields are in progress with scientists from other institutions. A service program is operated to make sure that the Resource can be used freely by all qualified and interested scientists. The new features of the Resource are made known by a regular series of workshops and symposia and seminars presented by the Resource staff.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR001838-04
Application #
3104012
Study Section
(SSS)
Project Start
1984-06-01
Project End
1990-05-31
Budget Start
1987-06-01
Budget End
1988-05-31
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Associated University-Brookhaven National Lab
Department
Type
DUNS #
City
Upton
State
NY
Country
United States
Zip Code
11973
Jones, K W; Bockman, R S; Bronner, F (1992) Microdistribution of lead in bone: a new approach. Neurotoxicology 13:835-41
Vittur, F; Tuniz, C; Paoletti, S et al. (1992) Elemental analysis of growth plate cartilage by synchrotron-radiation-induced X-ray emission (SRIXE). Biochem Biophys Res Commun 188:1010-7
Meshinchi, S; Stancato, L F; Gordon, B M et al. (1991) Purification of the endogenous glucocorticoid receptor stabilizing factor. Biochemistry 30:8617-22
Gordon, B M; Jones, K W; Hanson, A L et al. (1990) An X-ray microprobe facility using synchrotron radiation. Biol Trace Elem Res 26-27:133-41
Pounds, J G (1990) The role of cell calcium in current approaches to toxicology. Environ Health Perspect 84:7-15
Jones, K W; Schidlovsky, G; Burger, D E et al. (1990) Distribution of lead in human bone: III. Synchrotron x-ray microscope measurements. Basic Life Sci 55:281-6
Schidlovsky, G; Jones, K W; Burger, D E et al. (1990) Distribution of lead in human bone: II. Proton microprobe measurements. Basic Life Sci 55:275-80
Batuman, V; Wedeen, R P; Bogden, J D et al. (1989) Reducing bone lead content by chelation treatment in chronic lead poisoning: an in vivo X-ray fluorescence and bone biopsy study. Environ Res 48:70-5
Giauque, R D; Thompson, A C; Underwood, J H et al. (1988) Measurement of femtogram quantities of trace elements using an X-ray microprobe. Anal Chem 60:855-8
Jones, K W; Gordon, B M; Hanson, A L et al. (1988) X-ray fluorescence with synchrotron radiation. Ultramicroscopy 24:313-28

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