Conformational heterogeneity is emerging as a common feature of oligodeoxynucleotides adducted with chemical carcinogens. Spectroscopically, conformational heterogeneity introduces several problems. First, resonance signals arising from each conformation present in the sample must be individually resolved and identified. Second, conformational heterogeneity reduces the effective sample concentration. Third, methodology for structural refinement of these adducts is not well-developed. Ultra high field NMR provides an attractive method for examining conformationally labile samples because of its extraordinary resolving power and sensitivity, to help identify and assign resonances which arise from conformation exchange. We propose to take advantage of this to develop a better understanding of the conformational equilibria involved for styrene oxide and benzo[a]pyrene adducts of adenine, in an effort to correlate adduct structure with function.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR002301-16
Application #
6309198
Study Section
Project Start
2000-04-15
Project End
2005-02-28
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
16
Fiscal Year
2000
Total Cost
$7,533
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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