This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As Director of the NMR laboratory in the Research Resources Center at the University of Illinois at Chicago (UIC), I am responsible for assisting UICresearchers using our facilities. Recently new users have requested to do NMR experiments for which the NMR staff has little or no experience. Most of these new experiments analyze proteins, DNA and polysaccarides using inverse detection experiments in water.In order to develope the expertise necessary to perform these NMR experiments, I and selected UIC researchers will utilize your 500 MHz NMR spectrometer and staff at NMRFAM to develope the NMR protocols and procedures necessary for the successful completion of these experiments.Our NMR laboratory has a 500 MHz AVANCE NMR spectrometer very similar to your instrument, thus experience gained at NMRFAM will be readilytransferable to our equipment. Samples will be provided by UIC researchers so that results from NMRFAM and our NMR lab can be immediately compared.The procedures to be improved include:triple gradient autoshimming, acquisition and processing parameters for several 2D experiments including NOESY, ROESY AND TOCSY and aqueous sample preparation.The experiments include:HSQC, HNCO, HNCA, HNCOCA, HNCACB, CBCACONH, TOCSY-DIPSY AND HCCH-DIPSY.
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