This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Brazzein is a small, highly heat stable sweet protein. We have overexpressed and purified brazzein in E. coli. Our main goal is to determine the sweet sites on this small protein. In this study, we are using a non-sweet brazzein variant and would like to see if there are local or global changes due to the mutation. Furthermore, we would like to use the N15-NOESY data for future structure/function studies.
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