This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One of the main goals of NMRFAM is to implement, optimize and further develop pulse programs for the collection of data on high field instruments, equipped with cryogenic probes. Cryogenic probes are notoriously difficult in terms of water suppression and have more stringent power requirements than conventional probes. Hence, particular care is put in modifying experiments to improve handling of water magnetization and achieve better water suppression and often increase sensitivity for some experiments.Furthermore, we have put together and will continue to expand and develop a library of the most commonly used experiments used for studying proteins and RNA. These experiments have been optimized to yield the best possible sensitivity and spectral quality on our instruments, as well as, to minimize the time required by the user for setting them up.Finally, users of the facility often submit samples that require particular experiments to be implemented, tested and often need to be modified and customized in order to yield the expected results for their particular system.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR002301-25
Application #
8168962
Study Section
Special Emphasis Panel (ZRG1-BCMB-H (40))
Project Start
2010-03-01
Project End
2011-02-28
Budget Start
2010-03-01
Budget End
2011-02-28
Support Year
25
Fiscal Year
2010
Total Cost
$18,647
Indirect Cost
Name
University of Wisconsin Madison
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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