This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Obtain additional spectral information from representatives of the 100 important metabolites of Arabidopsis and populate the database. In the future, we will have a catalog containing the structures of the 10,000 or more'most-abundant smallmolecules in all human tissues. We will also be able to use this information to monitor their fluctuations in concentration in real time, as our bodies continually change. The purpose of this proposal is to take us one step closer to creating metabolomic technologies to fill this void. This proposal explores the use of stable isotope labeling of biological samples coupled with the analytical high sensitivity of mass spectrometry (MS) and the chemical selectivity of high-field NMR spectroscopy as enabling technologies for high-throughput analysis of metabolomes. We call this novel approach isotopeassisted metabolomics.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002301-26
Application #
8361203
Study Section
Special Emphasis Panel (ZRG1-BCMB-H (40))
Project Start
2011-03-01
Project End
2012-02-29
Budget Start
2011-03-01
Budget End
2012-02-29
Support Year
26
Fiscal Year
2011
Total Cost
$9,400
Indirect Cost
Name
University of Wisconsin Madison
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Bhute, Vijesh J; Ma, Yan; Bao, Xiaoping et al. (2016) The Poly (ADP-Ribose) Polymerase Inhibitor Veliparib and Radiation Cause Significant Cell Line Dependent Metabolic Changes in Breast Cancer Cells. Sci Rep 6:36061
Wessel, Sarah R; Cornilescu, Claudia C; Cornilescu, Gabriel et al. (2016) Structure and Function of the PriC DNA Replication Restart Protein. J Biol Chem 291:18384-96
Okuno, Yusuke; Cavagnero, Silvia (2016) Fluorescein: A Photo-CIDNP Sensitizer Enabling Hypersensitive NMR Data Collection in Liquids at Low Micromolar Concentration. J Phys Chem B 120:715-23
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Malecki, Marek; Sabo, Chelsea; Foorohar, Afsoon et al. (2016) Novel paradigm for immunotherapy of breast cancer by engaging prophylactic immunity against hepatitis B. Clin Transl Med 5:32
Kim, Sophia; Natesan, Senthil; Cornilescu, Gabriel et al. (2016) Mechanism of Histone H3K4me3 Recognition by the Plant Homeodomain of Inhibitor of Growth 3. J Biol Chem 291:18326-41
Berry, Robert E; Muthu, Dhanasekaran; Yang, Fei et al. (2015) NMR studies of the dynamics of high-spin nitrophorins: comparative studies of NP4 and NP2 at close to physiological pH. Biochemistry 54:221-39
Bothe, Jameson R; Tonelli, Marco; Ali, Ibrahim K et al. (2015) The Complex Energy Landscape of the Protein IscU. Biophys J 109:1019-25
Callaci, Sandhya; Morrison, Kylee; Shao, Xiangqiang et al. (2015) Phosphoregulation of the C. elegans cadherin-catenin complex. Biochem J 472:339-52
Bhute, Vijesh J; Palecek, Sean P (2015) Metabolic responses induced by DNA damage and poly (ADP-ribose) polymerase (PARP) inhibition in MCF-7 cells. Metabolomics 11:1779-1791

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