This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our purpose in this project is to design an easy-to-implement scheme for fluorescence microscopy with high spatial resolution and fluorescence enhancement properties based on the resonant excitation of 2D-PhC guided modes. It is well known that the interaction of an external radiation with a periodically nanostructure such as a PhC affects the far field propagation of electromagnetic waves. In brief, the periodicity allows for phase-matching of evanescent waves in the near field region to localized leaky modes supported by the PhC. Thus, the external excitation of leaky modes is associated with a total reflection phenomenon for a resonant wavelength. However, the light does not reflect abruptly;and an illuminated region called the evanescent wave region, is present on the interface between the PhC and the medium (typically air or water) where the intensity decays exponentially from the PhC surface. Using this PhC's property and the fluorescence enhancement shown previously [Estrada et al, 2010. Optics Express, Vol. 18, Issue 4, pp. 3693-3699 (2010)] we are designing new substrates for fluorescence microscopy.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR003155-26
Application #
8365767
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Project Start
2011-08-20
Project End
2012-06-30
Budget Start
2011-08-20
Budget End
2012-06-30
Support Year
26
Fiscal Year
2011
Total Cost
$232,079
Indirect Cost
Name
University of California Irvine
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Liang, Elena I; Mah, Emma J; Yee, Albert F et al. (2017) Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography. Integr Biol (Camb) 9:145-155
Digiacomo, Luca; Digman, Michelle A; Gratton, Enrico et al. (2016) Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles. Acta Biomater 42:189-198
Malacrida, Leonel; Astrada, Soledad; Briva, Arturo et al. (2016) Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. Biochim Biophys Acta 1858:2625-2635
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2016) Self-assisted optothermal trapping of gold nanorods under two-photon excitation. Methods Appl Fluoresc 4:035003
Golfetto, Ottavia; Hinde, Elizabeth; Gratton, Enrico (2015) The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells. Methods Mol Biol 1232:273-90
Willenberg, Rafer; Steward, Oswald (2015) Nonspecific labeling limits the utility of Cre-Lox bred CST-YFP mice for studies of corticospinal tract regeneration. J Comp Neurol 523:2665-82
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2015) Spectral properties and dynamics of gold nanorods revealed by EMCCD-based spectral phasor method. Microsc Res Tech 78:283-93
Scarlata, Suzanne; Golebiewska, Urszula (2014) Linking alpha-synuclein properties with oxidation: a hypothesis on a mechanism underling cellular aggregation. J Bioenerg Biomembr 46:93-8
Pang, Yan Ling Joy; Poruri, Kiranmai; Martinis, Susan A (2014) tRNA synthetase: tRNA aminoacylation and beyond. Wiley Interdiscip Rev RNA 5:461-80
Sharma, Himanshu; Digman, Michelle A; Felsinger, Natasha et al. (2014) Enhanced emission of fluorophores on shrink-induced wrinkled composite structures. Opt Mater Express 4:753-763

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