Monoclonal Antibody Treatment of Brain Tumors: an MRI Study: Glioma

Leavitt, Marc

Abstract

The overall goal of this project is to assess the utility of MRI in evaluating the effectiveness of various treatment protocols for brain tumors. Currently investigated treatment consists of intratumoral infusion of murine monoclonal antibody (MAb) which is reactive to a tumor-specific antigen normally only expressed by trophoblasts, embronic and fetal cells. Permanently implanted plastic guide cannulae were positioned stereotaxically 1 mm deep to the skull surface in rats. C-6 glyoma cells were infused into through the implanted guide cannula. The rats were imaged by MRI immediately before the start of the intratumoral treatment and following completion of treatment. Monoclonal antibody treatment was administered through the implanted guide cannula with the help of an osmotic minipump subcutaneously implanted in the rat. We have to date performed post-treatment MRI examinations in antibody treated rats that survived long-term. These MR images in comparison to each rat's pre-treatment MRIs reveal a complete elimination of the tumor. This finding has been confirmed by histology. In contrast, post-treatment MRIs of rats infused with a control substance showed pronounced tumor enlargement. Our studies have shown that MRI can serve as a valuable tool in the assessment of the progression of a brain glioma under various treatment protocols.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Center for Research Resources (NCRR)
• Type: Biotechnology Resource Grants (P41)
• Project #: 5P41RR003631-09
• Application #: 5224629
• Support Year: 9
• Fiscal Year: 1996

Publications

Ramachandran, Suchitra; Meyer, Travis; Olson, Carl R (2016) Prediction suppression in monkey inferotemporal cortex depends on the conditional probability between images. J Neurophysiol 115:355-62

Meyer, Travis; Walker, Christopher; Cho, Raymond Y et al. (2014) Image familiarization sharpens response dynamics of neurons in inferotemporal cortex. Nat Neurosci 17:1388-94

Hall, Nathan; Colby, Carol (2014) S-cone visual stimuli activate superior colliculus neurons in old world monkeys: implications for understanding blindsight. J Cogn Neurosci 26:1234-56

Subramanian, Janani; Colby, Carol L (2014) Shape selectivity and remapping in dorsal stream visual area LIP. J Neurophysiol 111:613-27

Berdyyeva, Tamara K; Olson, Carl R (2014) Intracortical microstimulation of supplementary eye field impairs ability of monkeys to make serially ordered saccades. J Neurophysiol 111:1529-40

Meyer, Travis; Ramachandran, Suchitra; Olson, Carl R (2014) Statistical learning of serial visual transitions by neurons in monkey inferotemporal cortex. J Neurosci 34:9332-7

Hall, Nathan; Colby, Carol (2013) Psychophysical definition of S-cone stimuli in the macaque. J Vis 13:

Leathers, Marvin L; Olson, Carl R (2012) In monkeys making value-based decisions, LIP neurons encode cue salience and not action value. Science 338:132-5

Meyer, Travis; Olson, Carl R (2011) Statistical learning of visual transitions in monkey inferotemporal cortex. Proc Natl Acad Sci U S A 108:19401-6

Berdyyeva, Tamara K; Olson, Carl R (2011) Relation of ordinal position signals to the expectation of reward and passage of time in four areas of the macaque frontal cortex. J Neurophysiol 105:2547-59