This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.During the previous funding period, we have invested considerable effort and time in merging tetracysteine tags with other reporter systems to generate hybrids with enhanced performance for both LM and EM detection. This report covers the progress we made in: (1) Enhancing the sensitivity and the signal/noise ratio of protein detection with biarsenicals. (2) Detecting nucleic acids and interacting proteins in correlated LM/EM studies. (3) Developing a photo-reversible protein aggregation tag for functional studies. (4) Labeling with biarsenicals after fixation. (5) Developing methods for the simultaneous preservation of fluorescent signals, ultrastructure and antigenicity. (6) Developing multiple labeling strategies using EEL spectroscopy.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR004050-20
Application #
7722409
Study Section
Special Emphasis Panel (ZRG1-CDF-2 (40))
Project Start
2008-05-01
Project End
2009-04-30
Budget Start
2008-05-01
Budget End
2009-04-30
Support Year
20
Fiscal Year
2008
Total Cost
$126,828
Indirect Cost
Name
University of California San Diego
Department
Neurosciences
Type
Schools of Medicine
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
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