This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our previous work with actively stained mouse brains has allowed us to see the cytoarchitecture down to 20 microns. But, our understanding of the sources of contrast at this level of resolution is quite primative. The goal of this project is to understand more fully the sources of contrast of the cytoarchitecture in the actively stained mouse brain using the 9T MR magnet. 1. We will acquire quantitative T1 and T2 maps of the mouse brain at 40 microns with accompanying higher resolution spin echo (T1-weighted) images for definition of the cytoarchitecture. 2. We will histologically section the same brains and register the resulting datasets to produce 3D optical sectioned data with Nissl and Cresyl violet. 3. MR and optical sections will be registered into single volumes. 4. The resulting data will be used to define the specific sources of contrast in the most critical regions of the actively stained brain.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR005959-22
Application #
8363148
Study Section
Special Emphasis Panel (ZRG1-SBIB-P (40))
Project Start
2011-07-01
Project End
2012-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
22
Fiscal Year
2011
Total Cost
$18,408
Indirect Cost
Name
Duke University
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
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