This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During the past decade, hyperpolarized (HP) 129Xe has found application in biomedical MRI with exciting results. Typically, the gas is introduced into the organism by inhalation, which is an efficient strategy for imaging the gas space of the lungs. Due to the alveolar gas exchange, a small amount of inhaled HP 129Xe enters the pulmonary blood and is delivered to other organs. This has already been exploited to study brain perfusion. More efficient methods for the local delivery of xenon to other target organs may include pre-dissolving of the HP gas in a suitable liquid or the production of microbubbles with subsequent injection into the blood stream. For initial experiments, we will use isotopically enriched 129Xe (82%), Intralipid 30% (Pharmacia, Clayton, NC) and a 'shaker'as described in M?ller et al. MRM 41, 1058 (1999) to reproduce previous experiments serving as a reference. The search for other vehicles for local delivery will include dissolving of HP 129Xe in fully oxygenated blood or plasma and dissolving of HP 129Xe in deuterated saline solution. Although the solubility of xenon in these liquids is significantly less than that of lipid emulsions, they are of interest because Xe will be released to the alveolar gas phase upon passing through the lungs, which can be exploited for perfusion imaging if RF excitation is selectively applied to the gas phase resonance. In addition, such systems may be tolerated better during prolonged infusion experiments. Experiments with deuterated saline are of further interest one may expect a drasticallyprolonged 129Xe T1. Insead of the previously used 'shaker'for dissolving HP 129Xe, another strategy will involve hollow-fiber membranes to improve the process of dissolving. This technique may also hold a potential for continuous infusion of the HP gas carrier. For comparison, experiments involving direct injection of small amounts of HP 129Xe into a vein will also be investigated. Regarding the Ostwald solubility (L) of Xe in water, roughly 0.1 mL of gas may be injected per mL of blood. If f is the venous blood flow in mL/sec, HP gas may be injected continuously at a rate of L x f provided that the dissolving process is sufficently rapid in vivo and the gas is quantitatively released to the alveoli.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-SBIB-P (40))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Duke University
Schools of Medicine
United States
Zip Code
Tang, Xinyan; Jing, Liufang; Richardson, William J et al. (2016) Identifying molecular phenotype of nucleus pulposus cells in human intervertebral disc with aging and degeneration. J Orthop Res 34:1316-26
Hodgkinson, Conrad P; Bareja, Akshay; Gomez, José A et al. (2016) Emerging Concepts in Paracrine Mechanisms in Regenerative Cardiovascular Medicine and Biology. Circ Res 118:95-107
Schmeckpeper, Jeffrey; Verma, Amanda; Yin, Lucy et al. (2015) Inhibition of Wnt6 by Sfrp2 regulates adult cardiac progenitor cell differentiation by differential modulation of Wnt pathways. J Mol Cell Cardiol 85:215-25
Roos, Justus E; McAdams, Holman P; Kaushik, S Sivaram et al. (2015) Hyperpolarized Gas MR Imaging: Technique and Applications. Magn Reson Imaging Clin N Am 23:217-29
He, Mu; Robertson, Scott H; Kaushik, S Sivaram et al. (2015) Dose and pulse sequence considerations for hyperpolarized (129)Xe ventilation MRI. Magn Reson Imaging 33:877-85
Huang, Lingling; Walter, Vonn; Hayes, D Neil et al. (2014) Hedgehog-GLI signaling inhibition suppresses tumor growth in squamous lung cancer. Clin Cancer Res 20:1566-75
Huang, Jing; Guo, Jian; Beigi, Farideh et al. (2014) HASF is a stem cell paracrine factor that activates PKC epsilon mediated cytoprotection. J Mol Cell Cardiol 66:157-64
Yuan, Ying; Gilmore, John H; Geng, Xiujuan et al. (2014) FMEM: functional mixed effects modeling for the analysis of longitudinal white matter Tract data. Neuroimage 84:753-64
He, Mu; Kaushik, S Sivaram; Robertson, Scott H et al. (2014) Extending semiautomatic ventilation defect analysis for hyperpolarized (129)Xe ventilation MRI. Acad Radiol 21:1530-41
Liu, Chunlei; Li, Wei (2013) Imaging neural architecture of the brain based on its multipole magnetic response. Neuroimage 67:193-202

Showing the most recent 10 out of 239 publications