This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Biological minerals such as vertebrate bone and tooth exhibit remarkable levels of hierarchy that are controlled over multiple length scales to produce superior mechanical properties compared to their individual building blocks. The process of mineralization involves the deposition of a protein scaffold upon which controlled crystallization of carbonated apatite occurs. In an effort to create biomimetic materials for use with mineralized tissues our lab is exploring the use of peptide amphiphiles as a synthetic scaffold. These molecules have been utilized by the Stupp laboratory to achieve a collection of bioengineering goals: as cell and tissue artificial scaffolds;as biomaterials that direct stem cell differentiation cell migration cellular response and tissue regeneration after injury;as vehicles for drug cell peptide and protein delivery [8-12];and as materials that induce biomineralization for bone and tooth enamel formation [13-15]. The proposed experiments focus on understanding the supramolecular structures that these PA molecules create. Additionally we plan to utilize PA scaffolds as a structural matrix for supporting nucleation and growth of biologically relevant mineral and characterize the inorganic-organic relationships at the nanoscale using X-ray diffraction. Understanding the assembly of peptide amphiphiles and interactions occurring at the organic-inorganic interface in biomineralized structures can elucidate structure-function relationships achieved by biology and enable the development of novel intelligent materials.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BCMB-P (40))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Chicago
Schools of Medicine
United States
Zip Code
Fournier, Bertrand; Sokolow, Jesse; Coppens, Philip (2016) Analysis of multicrystal pump-probe data sets. II. Scaling of ratio data sets. Acta Crystallogr A Found Adv 72:250-60
Mariette, Céline; Guérin, Laurent; Rabiller, Philippe et al. (2015) The creation of modulated monoclinic aperiodic composites in n-alkane/urea compounds. Z Kristallogr Cryst Mater 230:5-11
Sampath, Sujatha; Yarger, Jeffery L (2015) Structural hysteresis in dragline spider silks induced by supercontraction: An x-ray fiber micro-diffraction study. RSC Adv 5:1462-1473
Weingarten, Adam S; Kazantsev, Roman V; Palmer, Liam C et al. (2015) Supramolecular Packing Controls Hâ‚‚ Photocatalysis in Chromophore Amphiphile Hydrogels. J Am Chem Soc 137:15241-6
Coppens, Philip; Fournier, Bertrand (2015) On the scaling of multicrystal data sets collected at high-intensity X-ray and electron sources. Struct Dyn 2:064101
Pfoh, Roland; Pai, Emil F; Saridakis, Vivian (2015) Nicotinamide mononucleotide adenylyltransferase displays alternate binding modes for nicotinamide nucleotides. Acta Crystallogr D Biol Crystallogr 71:2032-9
Liang, Wenguang G; Ren, Min; Zhao, Fan et al. (2015) Structures of human CCL18, CCL3, and CCL4 reveal molecular determinants for quaternary structures and sensitivity to insulin-degrading enzyme. J Mol Biol 427:1345-58
Tucker, Chauncey L; Jones, Justin A; Bringhurst, Heidi N et al. (2014) Mechanical and physical properties of recombinant spider silk films using organic and aqueous solvents. Biomacromolecules 15:3158-70
Albertson, Amy E; Teule, Florence; Weber, Warner et al. (2014) Effects of different post-spin stretching conditions on the mechanical properties of synthetic spider silk fibers. J Mech Behav Biomed Mater 29:225-34
Reddy, Vijay S; Nemerow, Glen R (2014) Reply to Campos: Revised structures of adenovirus cement proteins represent a consensus model for understanding virus assembly and disassembly. Proc Natl Acad Sci U S A 111:E4544-5

Showing the most recent 10 out of 94 publications