This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Oxidative stress is linked with neurodegenerative diseases, such as Parkinson's or Alzheimer's, and brain antioxidants (glutathione, GSH; ascorbate, Asc) have a major protective function. Non-invasive detection and quantification of these major antioxidants in human brain is possible exclusively using in vivo NMR spectroscopy. To increase the reliability of quantification, a double editing pulse sequence was developed for simultaneous detection of both antioxidants (Terpstra et al. 2006b). Detection of antioxidant levels in the human brain is unique for the high field due to increased spectra resolution. In addition, spectral parameters of oxidized forms of ascorbate (DHA) and glutathione (GSSG) were measured at physiological conditions to estimate their contribution in GSH and Asc quantification. It was demonstrated that quantification of reduced forms of antioxidants was not affected by their oxidized forms, because of spectral differences between Asc and DHA and between GSH and GSSG and the spectral selectivity of the editing method.
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