This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Type I collagen is the single most abundant collagen in the animal kingdom and a critical part of most mammalian connective tissues. Type II collagen is a crucial component of mammalian cartilage, inter-vertebral discs and other tissues during their development, yet relatively little is known concerning the organization of these two collagen types at the sub-fibrillar level. Fortunately, the fibrillar type I and II collagen's found within rat tail tendon and in lamprey tissues are somewhat crystalline, a property that will allow an investigation of collagen molecular structure through X-ray fiber diffraction. Diffraction patterns obtained from both rat tail tendon and several lamprey tissues contain information regarding the sub-fibrillar organization of collagen chains, the specific level of interest to this study. Previously, such data (collected at BioCAT) was used to solve the 3D structure of type I collagen to 1.1 nm, revealing important constraints on the mechanism by which collagenase (and other EM active molecules) bind the collagen chains. Subsequent developments in cryo-freezing techniques will be used to extend the resolution of this structure, whilst data is collected for type II collagen using the newly developed micro-focus capabilities of the BioCAT beamline to similarly solve its structure using MIR.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008630-15
Application #
8168617
Study Section
Special Emphasis Panel (ZRG1-BCMB-E (40))
Project Start
2010-01-01
Project End
2010-12-31
Budget Start
2010-01-01
Budget End
2010-12-31
Support Year
15
Fiscal Year
2010
Total Cost
$64,740
Indirect Cost
Name
Illinois Institute of Technology
Department
Other Basic Sciences
Type
Schools of Arts and Sciences
DUNS #
042084434
City
Chicago
State
IL
Country
United States
Zip Code
60616
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