ESI-triple quadrupole tandem mass spectrometry (MS/MS) has previously been applied to glycosidic linkage assignment in carbohydrates by combining permethylation with alkali metal cation adduction in positive mode ionization and has provided both sensitivity and extensive glycosidic characterization. However, the linkage identification is often compromised by the presence of N-acetylated amino sugars or sialic acids and by increasing molecular size, factors which tend to promote glycosidic fragmentation over the ring-opening pathways required for linkage identification. Previous investigators have assigned linkages in disaccharides by negative mode ionization and low energy collisions. In this study, linkage analysis by negative mode electrospray ionization (ESI) and triple quadrupole MS/MS is extended to larger oligosaccharides. Linkage identification proceeds by specific eliminations from the reducing end. Although the initial site of deprotonation is not required to b e the reducing terminal, glycosidic linkages of oligosaccharides without a free reducing end cannot generally be determined.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR010888-03
Application #
6123244
Study Section
Project Start
1998-07-01
Project End
1999-06-30
Budget Start
Budget End
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Boston University
Department
Type
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118
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